Human CD272 Protein, His Tag_天津卡梅德科技发展有限公司

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Human CD272 Protein, His Tag

Human CD272 Protein, His Tag

产品编号

KMP2407

别名

B和T淋巴细胞衰减因子, B- and T-Lymphocyte Attenuator, CD272

规格

50ug
100ug
200ug

DATASHEET MSDS

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产品介绍

Catalog Number	KMP2407
Alias	B和T淋巴细胞衰减因子, B- and T-Lymphocyte Attenuator, CD272
Size	50ug, 100ug, 200ug
Product Description	The Human CD272 Protein(KMP2407) is produced in HEK293 Cells and the target gene encoding Lys31-Leu150 is expressed with a 6His tag at the C-terminus.
Molecular Name	CD272
Product Introduction	CD272（BTLA）：免疫球蛋白超家族（IgSF）成员，通过跨膜形式参与T细胞活化的负向调控。
Molecular Weight	13.86 kDa
Expression System	HEK293 Cells
Species	Human
Purity	>95%
SDS-PAGE
Purification	Affinity Purification
Uniprot ID	Q400G7
Storage Condition	Aliquot and store at -20℃ to -80℃. Avoid repeated freezing and thawing cycles.
Formulation	20mM PB, 150mM NaCl, pH7.4
Shipping Condition	In general, the proteins are provided as lyophilized powder which are shipped at ambient temperature. They are shipped out in dry ice if supplied in liquid form.
Background	B- and T-Lymphocyte Attenuator(BTLA) is a single-pass type I membrane protein containing 1 Ig-like V-type(immunoglobulin-like) domain. BTLA expression is induced during activation of T cells, and BTLA remains expressed on Th1 cells but not Th2 cells. Like PD1 and CTLA4, BTLA interacts with a B7 homolog, B7H4. However, unlike PD-1 and CTLA-4, BTLA displays T-Cell inhibition via interaction with tumor necrosis family receptors(TNF-R), not just the B7 family of cell surface receptors. BTLA is a lymphocyte inhibitory receptor that inhibits lymphocytes during immune response. BTLA also is a ligand for tumor necrosis factor(receptor) superfamily, member 14(TNFRSF14), also known as herpes virus entry mediator(HVEM). BTLA-HVEM complexes negatively regulate T-cell immune responses.
Endotoxin	<1.0 EU/ug determined by the LAL method
Product Declaration	该产品仅供科研使用，不可直接用于人体或注射。

FAQ

如何提高重组蛋白的可溶性表达？

可优化表达条件（如降低诱导温度、调整IPTG浓度）、使用促溶标签（如SUMO、GST）、共表达分子伴侣，或尝试不同宿主系统（如哺乳动物细胞或昆虫细胞）以提高可溶性蛋白产量。
重组蛋白纯化常用的方法有哪些？

常用的纯化方法包括亲和层析（如His-Tag、GST-Tag）、离子交换层析、疏水相互作用层析（HIC）和凝胶过滤层析。具体方法取决于蛋白特性和标签类型。

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