| Catalog Number | KRP226 |
| Alias | 骨形态发生蛋白4,BMP-4,BMP4,BMP2B,BMP-2B,BMP2B1,Bone morphogenetic protein 4,Bone morphogenetic protein 2B,Bmp-4,Dvr-4 |
| Size | 20ug, 50ug, 100ug, 200ug, 1mg |
| Product Description | Mouse Bmp4(293-408 aa) |
| Molecular Name | Bmp4 |
| Product Introduction | 骨形态发生蛋白4是一种分泌蛋白，通过激活特定信号通路，诱导间充质干细胞向成骨细胞分化，是骨与软骨形成的关键诱导因子。 |
| Molecular Weight | 12 kDa |
| Expression System | HEK293F Cells |
| Species | Mouse |
| Concentration | 联系销售经理获得最新批次的浓度 |
| Purity | ≥95% |
| Purification | Affinity Purification |
| Uniprot ID | P21275 |
| Storage Condition | Store the product under sterile conditions after opening at -80℃ for 12 months. Store the lyophilized powder at -20℃. Avoid repeated freeze-thaw cycles. |
| Formulation | Supplied in PBS |
| Shipping Condition | This product is shipped on ice packs or dry ice. |
| Background | Growth factor of the TGF-beta superfamily that plays essential roles in many developmental processes, including neurogenesis, vascular development, angiogenesis and osteogenesis (PubMed:10049358, PubMed:14973287, PubMed:15206957). Acts in concert with PTHLH/PTHRP to stimulate ductal outgrowth during embryonic mammary development and to inhibit hair follicle induction (PubMed:17301089). Initiates the canonical BMP signaling cascade by associating with type I receptor BMPR1A and type II receptor BMPR2. Once all three components are bound together in a complex at the cell surface, BMPR2 phosphorylates and activates BMPR1A. In turn, BMPR1A propagates signal by phosphorylating SMAD1/5/8 that travel to the nucleus and act as activators and repressors of transcription of target genes. Positively regulates the expression of odontogenic development regulator MSX1 via inducing the IPO7-mediated import of SMAD1 to the nucleus (PubMed:34995814). Required for MSX1-mediated mesenchymal molar tooth bud development beyond the bud stage, via promoting Wnt signaling (PubMed:27713059, PubMed:8898217). Acts as a positive regulator of odontoblast differentiation during mesenchymal tooth germ formation, expression is repressed during the bell stage by MSX1-mediated inhibition of CTNNB1 signaling (PubMed:29148101). Able to induce its own expression in dental mesenchymal cells and also in the neighboring dental epithelial cells via an MSX1-mediated pathway (PubMed:8898217). Can also signal through non-canonical BMP pathways such as ERK/MAP kinase, PI3K/Akt or SRC cascades. For example, induces SRC phosphorylation which, in turn, activates VEGFR2, leading to an angiogenic response (By similarity). {ECO:0000250|UniProtKB:P12644, ECO:0000269|PubMed:10049358, ECO:0000269|PubMed:14973287, ECO:0000269|PubMed:15206957, ECO:0000269|PubMed:17301089, ECO:0000269|PubMed:27713059, ECO:0000269|PubMed:29148101, ECO:0000269|PubMed:34995814, ECO:0000269|PubMed:8898217}. |
| Endotoxin | < 0.1 EU per ug |
| Biological Activity | Measured by its ability to induce alkaline phosphatase production by ATDC5 mouse chondrogenic cells. The ED50 forthis effect is 5- 40 ng/mL |
| Product Declaration | 该产品仅供科研使用，不可直接用于人体或注射。 |
蛋白可以在-80°C下储存以便长期保存。应分装并避免冻融循环，以保持蛋白的稳定性。
可以通过优化质粒设计、使用高效转染试剂、优化细胞培养条件以及使用蛋白折叠伴侣或密码子优化来提高蛋白表达效率。
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