Human CD326 Protein, His Tag, Avi Tag_天津卡梅德科技发展有限公司

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Human CD326 Protein, His Tag, Avi Tag

Human CD326 Protein, His Tag, Avi Tag

产品编号

KMP2272

别名

上皮细胞粘附分子, Epithelial Cell Adhesion Molecule, CD326

规格

50ug
100ug
200ug

DATASHEET MSDS

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产品介绍

Catalog Number	KMP2272
Alias	上皮细胞粘附分子, Epithelial Cell Adhesion Molecule, CD326
Size	50ug, 100ug, 200ug
Product Description	The Human CD326 Protein(KMP2272) is produced in HEK293 Cells and the target gene encoding Gln24-Lys265 is expressed with a 6His, Avi tag at the C-terminus.
Molecular Name	CD326
Product Introduction	CD326（EpCAM）：上皮细胞黏附分子，以跨膜形式参与细胞间相互作用和组织结构维持。
Molecular Weight	28.25 kDa
Expression System	HEK293 Cells
Species	Human
Purity	>95%
SDS-PAGE
Purification	Affinity Purification
Uniprot ID	P16422
Storage Condition	Aliquot and store at -20℃ to -80℃. Avoid repeated freezing and thawing cycles.
Formulation	20mM PB, 150mM NaCl, pH7.2
Shipping Condition	In general, the proteins are provided as lyophilized powder which are shipped at ambient temperature. They are shipped out in dry ice if supplied in liquid form.
Background	Epithelial Cell Adhesion Molecule(EpCAM) is a signal type I transmembrane glycoprotein that belongs to the EPCAM family. EpCAM is composed of an extracellular domain with one thyroglobulin type-1 domain, a transmembrane domain and a cytoplasmic domain. EpCAM is found on the surface of adenocarcinoma, but not on mesodermal or neural cell membranes. The EpCAM molecule has been shown to function as a homophilic Ca2+ independent adhesion molecule. It may act as a physical homophilic interaction molecule between intestinal epithelial cells(IECs) and intraepithelial lymphocytes(IELs) at the mucosal epithelium as an immunological barrier providing the first line of defense against infection. Defects in EPCAM are a cause of hereditary non-polyposis colorectal cancer type 8(HNPCC8) and diarrhea type 5(DIAR5). EpCAM plays a role in embryonic stem cells proliferation and differentiation; it up-regulates the expression of FABP5, MYC and Cyclin A and Cyclin E. It is highly and selectively expressed by undifferentiated embryonic stem cells.
Endotoxin	<1.0 EU/ug determined by the LAL method
Product Declaration	该产品仅供科研使用，不可直接用于人体或注射。

FAQ

如何避免重组蛋白降解？

可在纯化过程中加入蛋白酶抑制剂、低温操作，优化储存条件（如分装冻存于-80°C），并避免反复冻融。某些情况下，可选用蛋白酶缺陷型宿主菌减少降解风险。
如何检测重组蛋白的表达和纯度？

常用SDS-PAGE、Western Blot检测表达，BCA/Bradford法测浓度，HPLC或质谱分析纯度，生物活性实验（如ELISA、酶活检测）验证功能。

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