Mouse CD27 Protein, Fc Tag, His Tag_天津卡梅德科技发展有限公司

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Mouse CD27 Protein, Fc Tag, His Tag

Mouse CD27 Protein, Fc Tag, His Tag

产品编号

KMP2335

别名

肿瘤坏死因子受体超家族成员 7, CD27 antigen, CD27L receptor, T-cell activation antigen CD27

规格

50ug
100ug
200ug

DATASHEET MSDS

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产品介绍

Catalog Number	KMP2335
Alias	肿瘤坏死因子受体超家族成员 7, CD27 antigen, CD27L receptor, T-cell activation antigen CD27
Size	50ug, 100ug, 200ug
Product Description	The Mouse CD27 Protein(KMP2335) is produced in HEK293 Cells and the target gene encoding Pro24-Arg182 is expressed with a Fc, 6His tag at the C-terminus.
Molecular Name	CD27
Product Introduction	CD27：TNF受体超家族成员，通过跨膜形式参与T细胞活化、增殖和记忆形成。
Molecular Weight	18.62 kDa
Expression System	HEK293 Cells
Species	Mouse
Purity	>95%
Purification	Affinity Purification
Uniprot ID	P41272
Storage Condition	Aliquot and store at -20℃ to -80℃. Avoid repeated freezing and thawing cycles.
Formulation	PBS, pH7.4
Shipping Condition	In general, the proteins are provided as lyophilized powder which are shipped at ambient temperature. They are shipped out in dry ice if supplied in liquid form.
Background	Mouse CD27 is a single-pass type I membrane protein which is a member of the tumor necrosis factor receptor superfamily. CD27 is required for generation and long-term maintenance of T cell immunity. It binds to ligand CD70, and plays a key role in regulating B-cell activation and immunoglobulin synthesis. CD27 transduces signals that lead to the activation of NF-κB and MAPK8/JNK. Adaptor proteins TRAF2 and TRAF5 have been shown to mediate the signaling process of this receptor. CD27-binding protein(SIVA), a proapoptotic protein, can bind to this receptor and is thought to play an important role in the apoptosis induced by this receptor.
Endotoxin	<1.0 EU/ug determined by the LAL method
Product Declaration	该产品仅供科研使用，不可直接用于人体或注射。

FAQ

如何提高重组蛋白的可溶性表达？

可优化表达条件（如降低诱导温度、调整IPTG浓度）、使用促溶标签（如SUMO、GST）、共表达分子伴侣，或尝试不同宿主系统（如哺乳动物细胞或昆虫细胞）以提高可溶性蛋白产量。
重组蛋白纯化常用的方法有哪些？

常用的纯化方法包括亲和层析（如His-Tag、GST-Tag）、离子交换层析、疏水相互作用层析（HIC）和凝胶过滤层析。具体方法取决于蛋白特性和标签类型。

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