Endopeptidase K_天津卡梅德科技发展有限公司

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Endopeptidase K

Endopeptidase K

产品编号

EN010

别名

蛋白酶K ，Proteinase K，Endopeptidase K

规格

100mg
1g
1kg

DATASHEET MSDS

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产品介绍

Catalog Number	EN010
Alias	蛋白酶K ，Proteinase K，Endopeptidase K
Size	100mg, 1g, 1kg
Product Description	This product is derived from the recombinant protein expressed in yeast, which is encoded by a gene for Proteinase K from Engyodontium album that has been optimized by site-directed mutagenesis.
Molecular Name	Proteinase K
Product Introduction	Proteinase K 是一种丝氨酸蛋白酶，广泛用于分子生物学实验中水解各种蛋白质。
Expression System	Yeast
Species	Engyodontium album
Purity	≥95%
Purification	Affinity purification
Storage Condition	Lyophilized powder: store at 4°C. Liquid form: store at -20°C.
Formulation	稀释缓冲液：20mM Tris HCl，pH 7.4；存储缓冲液：20mM Tris HCl，50%甘油，pH值7.4
Shipping Condition	This product is shipped at ambient temperature or with gel packs.
Background	Proteinase K is a broad-spectrum serine protease initially discovered in 1974, isolated and purified from the fungus Engyodontium album. The enzyme is named after its ability to degrade keratin, and it primarily cleaves peptide bonds at the carboxyl terminus of aliphatic or aromatic hydrophobic amino acids.
Predicted Molecular Weight	29.3 kDa
Reconstitution	50% glycerol or 20 mM sodium acetate, pH 5.4
Product Declaration	该产品仅供科研使用，不可直接用于人体或注射。

FAQ

如何储存293F细胞中表达的蛋白？

蛋白可以在-80°C下储存以便长期保存。应分装并避免冻融循环，以保持蛋白的稳定性。
如何提高293F细胞中的蛋白表达效率？

可以通过优化质粒设计、使用高效转染试剂、优化细胞培养条件以及使用蛋白折叠伴侣或密码子优化来提高蛋白表达效率。

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