| Alias | 粒细胞集落刺激因子, Granulocyte Colony-Stimulating Factor, G-CSF, human G-CSF Protein, Pluripoietin, Filgrastim, Lenograstim, CSF3, C17orf33, GCSF |
| Catalog Number | KMP1619 |
| Molecular Name | G-CSF/CSF3 |
| Species | Human |
| Host | E.coli |
| Size | 500ug, 1mg |
| Protein Family | Other protein |
| Purity | > 95% as determined by Tris-Bis PAGE, > 95% as determined by HPLC |
| Concentration | 联系销售经理获得最新批次的浓度 |
| Purification | Affinity Purification |
| Endotoxin | Less than 0.001 ng/ug (0.01 EU/ug) as determined by LAL test. |
| Formulation | Lyophilized from a 0.2 μm filtered solution of 10mM HAc-NaAc, 150mM NaCl, 0.004% Tween 80, 5% Mannitol, pH 4.0. |
| Background | Human Granulocyte-Colony-Stimulating Factor (G-CSF) is 20 kD glycoprotein containing internal disulfide bonds. It induces the survival, proliferation, and differentiation of neutrophilic granulocyte precursor cells and it functionally activates mature blood neutrophils. Among the family of colony-stimulating factors, G-CSF is the most potent inducer of terminal differentiation to granulocytes and macrophages of leukemic myeloid cell lines. The synthesis of G-CSF can be induced by bacterial endotoxins, TNF, Interleukin-1, and GM-CSF. Prostaglandin E2 inhibits the synthesis of G-CSF. In epithelial, endothelial, and fibroblastic cells secretion of G-CSF is induced by Interleukin-17. |
| Application | SDS-PAGE, WB, ELISA |
| Recommended Dilution | ELISA: 1:100-1:1000 |
| Tag | No Tag |
| Cross-reactivity | No cross-reactivity with other antigens or proteins. |
| Predicted Molecular Weight | 18.8 kDa |
| Predicted N Terminus | Met |
| Reconstitution | Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water. |
| Storage Condition | Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles. |
| Remarks | 收到产品后请立即按保存条件储存；使用前短暂离心，使内容物集中于管底；避免反复冻融。 |
| Shipping Condition | In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise. |
| Stability | Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles. |
| Sequence | TPLGPASSLPQSFLLKCLEQVRKIQGDGAALQEKLCATYKLCHPEELVLLGHS LGIPWAPLSSCPSQALQLAGCLSQLHSGLFLYQGLLQALEGISPELGPTLDTL QLDVADFATTIWQQMEELGMAPALQPTQGAMPAFASAFQRRAGGVLVASHLQS FLEVSYRVLRHLAQP |
| Molecular Weight | The protein has a predicted MW of 18.8 kDa. the protein migrates to 16 kDa under reduced (R) condition based on Tris-Bis PAGE result. |
| Cross-reactivity | No cross-reactivity with other antigens or proteins. |
| Uniprot ID | P09919-2 |
| Note | 仅供科研使用 |
| ELISA IC50(ppb) | ≥ 1:1000 |
| Function | Human Granulocyte-Colony-Stimulating Factor (G-CSF) is 20 kD glycoprotein containing internal disulfide bonds. It induces the survival, proliferation, and differentiation of neutrophilic granulocyte precursor cells and it functionally activates mature blood neutrophils. Among the family of colony-stimulating factors, G-CSF is the most potent inducer of terminal differentiation to granulocytes and macrophages of leukemic myeloid cell lines. The synthesis of G-CSF can be induced by bacterial endotoxins, TNF, Interleukin-1, and GM-CSF. Prostaglandin E2 inhibits the synthesis of G-CSF. In epithelial, endothelial, and fibroblastic cells secretion of G-CSF is induced by Interleukin-17. |
| Product Declaration | 该产品仅供科研使用，不可直接用于人体或注射。 |
| Temperature Range | -20℃ ~ -80℃ |
| Solubility | 复溶后易溶于无菌去离子水或PBS缓冲液（pH 7.4）。 |
| Appearance | Lyophilized powder |
| Product Full Name | Recombinant Human G-CSF/CSF3 Protein |
| Preservative Solution | 10mM HAc-NaAc, 150mM NaCl, 0.004% Tween 80, 5% Mannitol, pH 4.0 |
| Usage Method | 冻干粉请按“复溶”说明用无菌去离子水复溶后使用，液体产品可直接使用；具体工作浓度请参考“推荐稀释比例”。 |
| Target | G-CSF/CSF3 |
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