Anti-Flag VHH, HRP

NAT101

Anti Flag VHH, HRP, Anti-DYKDDDDK VHH, HRP, Anti DYKDDDDK VHH, HRP, Flag Tag, DYKDDDDK Tag, 抗Flag纳米抗体-HRP, 抗DYKDDDDK纳米抗体-HRP

DATASHEET COA

产品介绍

Alias	Anti Flag VHH, HRP, Anti-DYKDDDDK VHH, HRP, Anti DYKDDDDK VHH, HRP, Flag Tag, DYKDDDDK Tag, 抗Flag纳米抗体-HRP, 抗DYKDDDDK纳米抗体-HRP
Catalog Number	NAT101
Product Description	The Anti-Flag VHH, HRP (NAT101) is a highly specific and affinity anti-Flag(DYKDDDDK) nano-antibody coupled with HRP, which can be used to detect the fusion protein containing Flag.
Molecular Name	Flag
Species	Alpaca
Host	Yeast
Size	50ug, 100ug, 200ug
Clonity	Monoclonal
Purity	>95% Determined by SDS-PAGE
Concentration	Batch to Batch
Purification	Affinity purification
Formulation	PBS, pH 7.4
Background	Nano-antibody is a kind of variable region of heavy chain antibody which is found by scientists in 1989 and naturally lacks light chain. Its molecular weight is only 15 ku, which is 1/10 of that of conventional antibody. HRP-coupled Flag nano-antibody has small molecular weight (~15 KDa), strong specificity, high affinity and good stability, and can be produced stably with high quality, thus avoiding the difference between batches of traditional antibodies.
Immunogen	Recombinant Flag Protein
Application	ELISA, WB
Recommended Dilution	WB/ELISA 1:5000-1:10000
Tag	HRP
Cross-reactivity	No cross-reactivity with other antigens or proteins.
Storage Condition	Store at -20℃. Avoid repeated freezing and thawing cycles.
Shipping Condition	Shipped on ice packs.
Product Declaration	For Research Use Only!
Temperature Range	-20ºC

FAQ

如何储存 Anti-Flag VHH, HRP 抗体？

抗体可以在-20°C下储存以便常规使用，或-80°C用于长期保存。应分装储存并尽量减少冻融次数，以维持抗体的结合活性和稳定性。
如何稀释 Anti-Flag VHH, HRP 抗体用于检测实验？

建议使用PBS, pH 7.4缓冲液，并添加载体蛋白进行稀释。具体的稀释比例需根据实验类型（如Western Blot、ELISA）进行优化。稀释后的抗体建议在当天使用。为避免活性下降，请避免反复冻融原液，并尽量减少稀释后抗体在光下的暴露时间。

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