ZIKV (strain Zika SPH2015) NS5 Protein, His Tag_天津卡梅德科技发展有限公司

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ZIKV (strain Zika SPH2015) NS5 Protein, His Tag

ZIKV (strain Zika SPH2015) NS5 Protein, His Tag

产品编号

KMP4929

别名

寨卡病毒非结构蛋白5, ZIKV NS5, Nonstructural Protein 5, ZIKV NS5 Protein

规格

50ug
100ug
200ug

DATASHEET MSDS

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产品介绍

Alias	寨卡病毒非结构蛋白5, ZIKV NS5, Nonstructural Protein 5, ZIKV NS5 Protein
Catalog Number	KMP4929
Product Description	The ZIKV (strain Zika SPH2015) NS5 Protein(KMP4929) is produced in Insect Cells. A DNA sequence encoding the Zika virus (ZIKV) (strain ZikaSPH2015) NS5 (ALU33341.1) (Lys2772-Leu3423) was expressed with a polyhistidine tag at the C-terminus.
Molecular Name	ZIKV NS5
Species	ZIKV
Host	Insect Cells
Size	50ug, 100ug, 200ug
Purity	>95% as determined by SDS-PAGE
Purification	Affinity purification
Endotoxin	<1.0 EU/ug determined by the LAL method
Formulation	PBS, pH7.4
Storage Condition	Aliquot and store at -20℃ to -80℃. Avoid repeated freezing and thawing cycles.
Shipping Condition	In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. If supplied as liquid, the proteins will be shipped with dry ice.

FAQ

Q1-NS5蛋白的功能是什么？

NS5是病毒RNA依赖的RNA聚合酶（RdRp），负责基因组复制，并具有甲基转移酶活性，是抗病毒药物（如核苷类似物）的靶点。
Q2-如何检测NS5的酶活性？

使用荧光底物（如NTP类似物）或体外RNA合成实验，通过凝胶电泳检测产物。
Q3-能否用于高通量药物筛选？

是的，适用于96/384孔板形式的酶活抑制实验，筛选RdRp或甲基转移酶抑制剂。
Q4-His标签是否影响蛋白功能？

标签位于C端，经活性验证不影响催化功能或底物结合。
Q5-储存稳定性如何？

添加10%甘油和1 mM DTT，-80℃保存可维持活性6个月以上。

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