SARS Spike Protein, mFc Tag_天津卡梅德科技发展有限公司

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SARS Spike Protein, mFc Tag

SARS Spike Protein, mFc Tag

产品编号

KMP4897

别名

冠状病毒刺突蛋白, SARS Spike, S Protein, SARS Spike Protein

规格

50ug
100ug
200ug

DATASHEET MSDS

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产品介绍

Catalog Number	KMP4897
Alias	冠状病毒刺突蛋白, SARS Spike, S Protein, SARS Spike Protein
Size	50ug, 100ug, 200ug
Product Description	The SARS Spike Protein(KMP4897) is produced in HEK293 Cells. A DNA sequence encoding the receptor binding domain (RBD) of SARS-CoV (isolate:WH20) spike (AAX16192.1) (Arg306-Phe527) was expressed with the Fc region of mouse IgG1 at the C-terminus.
Molecular Name	SARS Spike
Species	SARS
Host	HEK293 Cells
Purity	>95% as determined by SDS-PAGE
Purification	Affinity purification
Storage Condition	Aliquot and store at -20℃ to -80℃. Avoid repeated freezing and thawing cycles.
Formulation	PBS, pH7.4
Shipping Condition	In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. If supplied as liquid, the proteins will be shipped with dry ice.
Endotoxin	<1.0 EU/ug determined by the LAL method

FAQ

Q1-mFc标签的作用是什么？

小鼠Fc标签（mFc）可延长蛋白半衰期，便于Protein A/G纯化，并用于抗体依赖性功能研究。
Q2-该蛋白能否形成三聚体？

是的，经凝胶过滤层析（SEC）验证，以天然三聚体形式存在，模拟病毒表面构象。
Q3-是否适用于假病毒包装？

是的，与慢病毒载体共转染可生成SARS假病毒，用于中和抗体检测（需BSL-2条件）。
Q4-如何避免S蛋白的自发切割？

引入弗林蛋白酶切割位点突变（如RRAR→GSAS），维持Spike全长稳定性。
Q5-储存条件有何特殊要求？

添加0.01% Tween-20增强溶解度，-80℃保存，避免反复冻融导致聚集。

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