Influenza B (B/Phuket/3073/2013) NP Protein, His Tag_天津卡梅德科技发展有限公司

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Influenza B (B/Phuket/3073/2013) NP Protein, His Tag

Influenza B (B/Phuket/3073/2013) NP Protein, His Tag

产品编号

KMP4865

别名

乙型流感病毒核蛋白, Influenza B NP, Nucleoprotein, Influenza B NP Protein

规格

50ug
100ug
200ug

DATASHEET MSDS

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产品介绍

Alias	乙型流感病毒核蛋白, Influenza B NP, Nucleoprotein, Influenza B NP Protein
Catalog Number	KMP4865
Product Description	The Influenza B (B/Phuket/3073/2013) NP Protein(KMP4865) is produced in Insect Cells. A DNA sequence encoding the Influenza B (B/Phuket/3073/2013) nucleoprotein (translated amino acids of EPI1649065) (Met1-Tyr560) was expressed with a polyhistidine tag at the C-terminus.
Molecular Name	Influenza B NP
Species	Influenza B
Host	Insect Cells
Size	50ug, 100ug, 200ug
Purity	>90% as determined by SDS-PAGE
Purification	Affinity purification
Endotoxin	<1.0 EU/ug determined by the LAL method
Formulation	PBS, pH7.4
Background	The spike (S) glycoprotein of coronaviruses contains protrusions that will only bind to certain receptors on the host cell. Known receptors bind S1 are ACE2, angiotensin-converting enzyme 2, DPP4, dipeptidyl peptidase-4, APN, aminopeptidase N, CEACAM, carcinoembryonic antigen-related cell adhesion molecule 1, Sia, sialic acid, O-ac Sia, O-acetylated sialic acid. The spike is essential for both host specificity and viral infectivity. The term 'peplomer' is typically used to refer to a grouping of heterologous proteins on the virus surface that function together. The spike (S) glycoprotein of coronaviruses is known to be essential in the binding of the virus to the host cell at the advent of the infection process. It's been reported that SARS-CoV-2 (COVID-19 coronavirus, 2019-nCoV) can infect the human respiratory epithelial cells through interaction with the human ACE2 receptor. The spike protein is a large type I transmembrane protein containing two subunits, S1 and S2. S1 mainly contains a receptor binding domain (RBD), which is responsible for recognizing the cell surface receptor. S2 contains basic elements needed for the membrane fusion. The S protein plays key parts in the induction of neutralizing-antibody and T-cell responses, as well as protective immunity. The main functions for the Spike protein are summarized as: Mediate receptor binding and membrane fusion, Defines the range of the hosts and specificity of the virus, Main component to bind with the neutralizing antibody, Key target for vaccine design, Can be transmitted between different hosts through gene recombination or mutation of the receptor binding domain (RBD), leading to a higher mortality rate.
Storage Condition	Aliquot and store at -20℃ to -80℃. Avoid repeated freezing and thawing cycles.
Shipping Condition	In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. If supplied as liquid, the proteins will be shipped with dry ice.

FAQ

Q1-NP蛋白的生物学功能是什么？

NP包裹病毒RNA形成核糖核蛋白复合体（RNP），是病毒复制核心，也是T细胞免疫应答的主要靶标。
Q2-该毒株的临床重要性是什么？

B/Phuket/3073/2013是乙型流感Victoria谱系的代表株，其NP研究可支持诊断试剂开发及疫苗优化。
Q3-如何验证NP的RNA结合能力？

通过电泳迁移率变动分析（EMSA）或荧光偏振实验，检测其与病毒RNA探针的结合。
Q4-是否与其他流感病毒NP交叉反应？

乙型流感NP与甲型流感NP无交叉反应，但可能与乙型不同谱系（Yamagata/Victoria）存在部分交叉。
Q5-储存稳定性如何？

-80℃保存2年内活性稳定，反复冻融＞3次可能导致聚集，建议分装使用。

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