HSV2 (strain HG52)GP E Protein(ECD), His Tag_天津卡梅德科技发展有限公司

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HSV2 (strain HG52)GP E Protein(ECD), His Tag

HSV2 (strain HG52)GP E Protein(ECD), His Tag

产品编号

KMP4837

别名

单纯疱疹病毒包膜糖蛋白E, HSV2 GP E, Glycoprotein E, HSV2 GP E Protein

规格

50ug
100ug
200ug

DATASHEET MSDS

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产品介绍

Alias	单纯疱疹病毒包膜糖蛋白E, HSV2 GP E, Glycoprotein E, HSV2 GP E Protein
Catalog Number	KMP4837
Product Description	The HSV2 (strain HG52)GP E Protein(KMP4837) is produced in Insect Cells. A DNA sequence encoding the HSV 2 ((strain HG52) (HSV2)) gE/glycoprotein E (translated amino acids of P89475) (Met1-Arg414) was expressed with a polyhistidine tag at the C-terminus.
Molecular Name	HSV2 GP E
Species	HSV2
Host	Insect Cells
Size	50ug, 100ug, 200ug
Purity	>90% as determined by SDS-PAGE
Purification	Affinity purification
Endotoxin	<1.0 EU/ug determined by the LAL method
Formulation	PBS, pH7.4
Background	The influenza viral Hemagglutinin (HA) protein is a homotrimer with a receptor binding pocket on the globular head of each monomer.HA has at least 18 different antigens. These subtypes are named H1 through H18.HA has two functions. Firstly, it allows the recognition of target vertebrate cells, accomplished through the binding to these cells' sialic acid-containing receptors. Secondly, once bound it facilitates the entry of the viral genome into the target cells by causing the fusion of the host endosomal membrane with the viral membrane. The influenza virus Hemagglutinin (HA) protein is translated in cells as a single protein, HA, or hemagglutinin precursor protein. For viral activation, hemagglutinin precursor protein (HA) must be cleaved by a trypsin-like serine endoprotease at a specific site, normally coded for by a single basic amino acid (usually arginine) between the HA1 and HA2 domains of the protein. After cleavage, the two disulfide-bonded protein domains produce the mature form of the protein subunits as a prerequisite for the conformational change necessary for fusion and hence viral infectivity.
Storage Condition	Aliquot and store at -20℃ to -80℃. Avoid repeated freezing and thawing cycles.
Shipping Condition	In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. If supplied as liquid, the proteins will be shipped with dry ice.

FAQ

Q1-gE蛋白在HSV-2感染中的作用是什么？

gE与gI形成异源二聚体，参与细胞间传播和免疫逃逸（如Fcγ受体结合抑制抗体功能）。
Q2-ECD（胞外域）是否保留天然构象？

经圆二色光谱（CD）分析，其二级结构与天然gE一致，适合抗体表位研究。
Q3-能否用于疫苗开发？

是的，gE是亚单位疫苗的候选抗原，可诱导针对HSV-2传播的中和抗体。
Q4-His标签如何去除？

提供TEV蛋白酶切割位点定制服务，切割后需二次纯化（额外订购）。
Q5-储存条件有何要求？

溶于含5%甘油的PBS，-80℃保存；避免反复冻融，分装后使用。

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