HSV2 (strain 333) GP Protein, His Tag_天津卡梅德科技发展有限公司

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HSV2 (strain 333) GP Protein, His Tag

HSV2 (strain 333) GP Protein, His Tag

产品编号

KMP4834

别名

单纯疱疹病毒包膜糖蛋白, HSV2 GP, Glycoprotein, HSV2 GP Protein

规格

50ug
100ug
200ug

DATASHEET MSDS

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产品介绍

Alias	单纯疱疹病毒包膜糖蛋白, HSV2 GP, Glycoprotein, HSV2 GP Protein
Catalog Number	KMP4834
Product Description	The HSV2 (strain 333) GP Protein(KMP4834) is produced in Insect Cells. A DNA sequence encoding the HSV 2 (strain 333 (HSV2)) gB (translated amino acids of P06763) (Glu98-Ala730) was expressed with a polyhistidine tag at the C-terminus.
Molecular Name	HSV2 GP
Species	HSV2
Host	Insect Cells
Size	50ug, 100ug, 200ug
Purity	>95% as determined by SDS-PAGE
Purification	Affinity purification
Endotoxin	<1.0 EU/ug determined by the LAL method
Formulation	PBS, pH7.4
Background	The influenza viral Hemagglutinin (HA) protein is a homotrimer with a receptor binding pocket on the globular head of each monomer.HA has at least 18 different antigens. These subtypes are named H1 through H18.HA has two functions. Firstly, it allows the recognition of target vertebrate cells, accomplished through the binding to these cells' sialic acid-containing receptors. Secondly, once bound it facilitates the entry of the viral genome into the target cells by causing the fusion of the host endosomal membrane with the viral membrane. The influenza virus Hemagglutinin (HA) protein is translated in cells as a single protein, HA, or hemagglutinin precursor protein. For viral activation, hemagglutinin precursor protein (HA) must be cleaved by a trypsin-like serine endoprotease at a specific site, normally coded for by a single basic amino acid (usually arginine) between the HA1 and HA2 domains of the protein. After cleavage, the two disulfide-bonded protein domains produce the mature form of the protein subunits as a prerequisite for the conformational change necessary for fusion and hence viral infectivity.
Storage Condition	Aliquot and store at -20℃ to -80℃. Avoid repeated freezing and thawing cycles.
Shipping Condition	In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. If supplied as liquid, the proteins will be shipped with dry ice.

FAQ

Q1-GP蛋白在HSV-2感染中的作用是什么？

GP（糖蛋白复合物）参与病毒吸附和宿主细胞膜融合，是中和抗体的主要靶标。
Q2-333毒株的研究价值是什么？

该临床分离株广泛用于HSV-2致病机制研究，其GP蛋白可指导疫苗设计。
Q3-能否用于黏膜免疫研究？

是的，结合黏膜佐剂（如CpG）可通过阴道或直肠途径评估保护效果。
Q4-His标签是否影响GP的受体结合？

标签位于C端，经细胞感染实验验证不影响其与宿主受体的相互作用。
Q5-如何检测蛋白的糖基化修饰？

使用PNGase F酶切后SDS-PAGE分析迁移率变化，确认糖基化程度。

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