HCoV-HKU1 Nucleocapsid Protein, His Tag_天津卡梅德科技发展有限公司

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HCoV-HKU1 Nucleocapsid Protein, His Tag

HCoV-HKU1 Nucleocapsid Protein, His Tag

产品编号

KMP4832

别名

人类冠状病毒核衣壳蛋白, HCoV-HKU1 Nucleocapsid, N Protein, HCoV-HKU1 Nucleocapsid Protein

规格

50ug
100ug
200ug

DATASHEET MSDS

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产品介绍

Alias	人类冠状病毒核衣壳蛋白, HCoV-HKU1 Nucleocapsid, N Protein, HCoV-HKU1 Nucleocapsid Protein
Catalog Number	KMP4832
Product Description	The HCoV-HKU1 (HCoV-HKU1) Nucleocapsid Protein(KMP4832) is produced in E. coli. A DNA sequence encoding the Human coronavirus (HCoV-HKU1) Nucleocapsid (YP_173242.1) (Met1-Ala441) was expressed with a polyhistide tag at the N-terminus.
Molecular Name	HCoV-HKU1 Nucleocapsid
Species	HCoV-HKU1
Host	E. coli
Size	50ug, 100ug, 200ug
Purity	>95% as determined by SDS-PAGE
Purification	Affinity purification
Endotoxin	<1.0 EU/ug determined by the LAL method
Formulation	PBS, pH7.4
Background	The influenza viral Hemagglutinin (HA) protein is a homotrimer with a receptor binding pocket on the globular head of each monomer.HA has at least 18 different antigens. These subtypes are named H1 through H18.HA has two functions. Firstly, it allows the recognition of target vertebrate cells, accomplished through the binding to these cells' sialic acid-containing receptors. Secondly, once bound it facilitates the entry of the viral genome into the target cells by causing the fusion of the host endosomal membrane with the viral membrane. The influenza virus Hemagglutinin (HA) protein is translated in cells as a single protein, HA, or hemagglutinin precursor protein. For viral activation, hemagglutinin precursor protein (HA) must be cleaved by a trypsin-like serine endoprotease at a specific site, normally coded for by a single basic amino acid (usually arginine) between the HA1 and HA2 domains of the protein. After cleavage, the two disulfide-bonded protein domains produce the mature form of the protein subunits as a prerequisite for the conformational change necessary for fusion and hence viral infectivity.
Storage Condition	Aliquot and store at -20℃ to -80℃. Avoid repeated freezing and thawing cycles.
Shipping Condition	In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. If supplied as liquid, the proteins will be shipped with dry ice.

FAQ

Q1-HKU1 NP蛋白的临床意义是什么？

HKU1是导致儿童呼吸道感染的重要病原体，其NP蛋白可用于血清学诊断和病毒载量监测。
Q2-能否与其他冠状病毒NP蛋白区分检测？

是的，通过Western Blot或ELISA验证，其抗体与其他人类冠状病毒（如229E）NP无交叉反应。
Q3-如何避免蛋白降解？

添加1 mM PMSF和EDTA，分装后-80℃保存，避免多次冻融。
Q4-是否提供RNA结合实验数据？

可提供EMSA实验报告，展示NP与HKU1基因组RNA特异性结合。
Q5-该蛋白的表达系统是什么？

采用大肠杆菌表达系统，经变复性处理确保正确折叠。

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