HCoV-229E Nucleocapsid Protein, His Tag_天津卡梅德科技发展有限公司

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HCoV-229E Nucleocapsid Protein, His Tag

HCoV-229E Nucleocapsid Protein, His Tag

产品编号

KMP4830

别名

人类冠状病毒核衣壳蛋白, HCoV-229E Nucleocapsid, N Protein, HCoV-229E Nucleocapsid Protein

规格

50ug
100ug
200ug

DATASHEET MSDS

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产品介绍

Alias	人类冠状病毒核衣壳蛋白, HCoV-229E Nucleocapsid, N Protein, HCoV-229E Nucleocapsid Protein
Catalog Number	KMP4830
Product Description	The HCoV-229E (HCoV-229E) Nucleocapsid Protein(KMP4830) is produced in E. coli. A DNA sequence encoding the Human coronavirus (HCoV-229E) Nucleocapsid (NP_073556.1) (Met1-Asn389) was expressed with a polyhistide tag at the N-terminus.
Molecular Name	HCoV-229E Nucleocapsid
Species	HCoV-229E
Host	E. coli
Size	50ug, 100ug, 200ug
Purity	>80% as determined by SDS-PAGE
Purification	Affinity purification
Endotoxin	<1.0 EU/ug determined by the LAL method
Formulation	20mM Tris, 500mM NaCl, 10% Glycerol, pH8.0
Background	The influenza viral Hemagglutinin (HA) protein is a homotrimer with a receptor binding pocket on the globular head of each monomer.HA has at least 18 different antigens. These subtypes are named H1 through H18.HA has two functions. Firstly, it allows the recognition of target vertebrate cells, accomplished through the binding to these cells' sialic acid-containing receptors. Secondly, once bound it facilitates the entry of the viral genome into the target cells by causing the fusion of the host endosomal membrane with the viral membrane. The influenza virus Hemagglutinin (HA) protein is translated in cells as a single protein, HA, or hemagglutinin precursor protein. For viral activation, hemagglutinin precursor protein (HA) must be cleaved by a trypsin-like serine endoprotease at a specific site, normally coded for by a single basic amino acid (usually arginine) between the HA1 and HA2 domains of the protein. After cleavage, the two disulfide-bonded protein domains produce the mature form of the protein subunits as a prerequisite for the conformational change necessary for fusion and hence viral infectivity.
Storage Condition	Aliquot and store at -20℃ to -80℃. Avoid repeated freezing and thawing cycles.
Shipping Condition	In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. If supplied as liquid, the proteins will be shipped with dry ice.

FAQ

Q1-核衣壳蛋白的功能是什么？

包裹病毒RNA形成螺旋核衣壳，参与基因组包装和免疫调节（如干扰宿主干扰素应答）。
Q2-能否用于核酸检测试剂的开发？

是的，作为阳性对照或抗体配对靶标，用于RT-PCR或抗原检测试剂盒。
Q3-如何验证其RNA结合能力？

通过电泳迁移率变动分析（EMSA）或荧光偏振实验检测与RNA探针的结合。
Q4-是否与其他冠状病毒NP蛋白有交叉反应？

经Western Blot验证，与SARS-CoV-2 NP无交叉反应，特异性高。
Q5-储存稳定性如何？

-80℃保存2年活性稳定，反复冻融＞3次可能导致聚集。

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