HCV (subtype 1b, strain HC-J4) Envelope GP E1 Protein, His Tag_天津卡梅德科技发展有限公司

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HCV (subtype 1b, strain HC-J4) Envelope GP E1 Protein, His Tag

HCV (subtype 1b, strain HC-J4) Envelope GP E1 Protein, His Tag

产品编号

KMP4816

别名

丙型肝炎病毒包膜蛋白E1, HCV Envelope GP E1, Envelope 1, HCV Envelope GP E1 Protein

规格

50ug
100ug
200ug

DATASHEET MSDS

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产品介绍

Alias	丙型肝炎病毒包膜蛋白E1, HCV Envelope GP E1, Envelope 1, HCV Envelope GP E1 Protein
Catalog Number	KMP4816
Product Description	The HCV (subtype 1b, strain HC-J4) Envelope GP E1 Protein(KMP4816) is produced in HEK293 Cells. A DNA sequence encoding the envelope glycoprotein E1 of Hepatitis C virus (subtype 1b, strain HC-J4) (AAC15725.1) (Tyr192-Ile340) was expressed with a polyhistidine tag at the N-terminus.
Molecular Name	HCV Envelope GP E1
Species	HCV
Host	HEK293 Cells
Size	50ug, 100ug, 200ug
Purity	>95% as determined by SDS-PAGE
Purification	Affinity purification
Endotoxin	<1.0 EU/ug determined by the LAL method
Formulation	PBS, pH7.4
Background	The influenza viral Hemagglutinin (HA) protein is a homotrimer with a receptor binding pocket on the globular head of each monomer.HA has at least 18 different antigens. These subtypes are named H1 through H18.HA has two functions. Firstly, it allows the recognition of target vertebrate cells, accomplished through the binding to these cells' sialic acid-containing receptors. Secondly, once bound it facilitates the entry of the viral genome into the target cells by causing the fusion of the host endosomal membrane with the viral membrane. The influenza virus Hemagglutinin (HA) protein is translated in cells as a single protein, HA, or hemagglutinin precursor protein. For viral activation, hemagglutinin precursor protein (HA) must be cleaved by a trypsin-like serine endoprotease at a specific site, normally coded for by a single basic amino acid (usually arginine) between the HA1 and HA2 domains of the protein. After cleavage, the two disulfide-bonded protein domains produce the mature form of the protein subunits as a prerequisite for the conformational change necessary for fusion and hence viral infectivity.
Storage Condition	Aliquot and store at -20℃ to -80℃. Avoid repeated freezing and thawing cycles.
Shipping Condition	In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. If supplied as liquid, the proteins will be shipped with dry ice.

FAQ

Q1-E1蛋白与E2的功能协同性是什么？

E1与E2形成异源二聚体，共同介导病毒进入，其中E1可能参与膜融合过程的后期步骤。
Q2-亚型1b的临床特点是什么？

1b型在亚洲和欧洲常见，对部分DAAs（如NS5A抑制剂）敏感性较低，研究E1可为联合疗法提供靶点。
Q3-如何验证E1与E2的结合能力？

通过共免疫沉淀（Co-IP）或表面等离子共振（SPR）检测两者的相互作用强度。
Q4-His标签是否影响E1的膜锚定功能？

本产品为可溶性截短体（去除跨膜区），His标签不影响其与E2的相互作用。
Q5-能否用于疫苗载体设计？

需与E2共表达形成复合物，可搭载病毒载体（如腺病毒）诱导中和抗体应答。

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