HCV (HCV-1a) NS3 Protease Protein, GST Tag_天津卡梅德科技发展有限公司

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HCV (HCV-1a) NS3 Protease Protein, GST Tag

HCV (HCV-1a) NS3 Protease Protein, GST Tag

产品编号

KMP4814

别名

丙型肝炎病毒蛋白酶NS3, HCV NS3 Protease, Helicase Immunodominant Region Protein, HCV NS3 Protease Protein

规格

50ug
100ug
200ug

DATASHEET MSDS

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产品介绍

Alias	丙型肝炎病毒蛋白酶NS3, HCV NS3 Protease, Helicase Immunodominant Region Protein, HCV NS3 Protease Protein
Catalog Number	KMP4814
Product Description	The HCV (HCV-1a) NS3 Protease Protein(KMP4814) is produced in E. coli. A DNA sequence encoding the Hepatitis C virus (HCV)(serotype 1a, isolate H77) NS3 (NP_803144.1) (Thr1356-Thr1459) was expressed with a GST tag at the N-terminus.
Molecular Name	HCV NS3 Protease
Species	HCV
Host	E. coli
Size	50ug, 100ug, 200ug
Purity	>95% as determined by SDS-PAGE
Purification	Affinity purification
Endotoxin	<1.0 EU/ug determined by the LAL method
Formulation	PBS, pH7.4
Background	The influenza viral Hemagglutinin (HA) protein is a homotrimer with a receptor binding pocket on the globular head of each monomer.HA has at least 18 different antigens. These subtypes are named H1 through H18.HA has two functions. Firstly, it allows the recognition of target vertebrate cells, accomplished through the binding to these cells' sialic acid-containing receptors. Secondly, once bound it facilitates the entry of the viral genome into the target cells by causing the fusion of the host endosomal membrane with the viral membrane. The influenza virus Hemagglutinin (HA) protein is translated in cells as a single protein, HA, or hemagglutinin precursor protein. For viral activation, hemagglutinin precursor protein (HA) must be cleaved by a trypsin-like serine endoprotease at a specific site, normally coded for by a single basic amino acid (usually arginine) between the HA1 and HA2 domains of the protein. After cleavage, the two disulfide-bonded protein domains produce the mature form of the protein subunits as a prerequisite for the conformational change necessary for fusion and hence viral infectivity.
Storage Condition	Aliquot and store at -20℃ to -80℃. Avoid repeated freezing and thawing cycles.
Shipping Condition	In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. If supplied as liquid, the proteins will be shipped with dry ice.

FAQ

Q1-NS3蛋白酶的功能是什么？

NS3与NS4A形成复合物，切割HCV多蛋白前体，生成功能性非结构蛋白，是DAAs（如特拉匹韦）的主要靶标。
Q2-GST标签的作用是什么？

GST（谷胱甘肽S-转移酶）标签便于谷胱甘肽亲和层析纯化，并可能增强蛋白可溶性。
Q3-如何检测NS3的酶活性？

使用荧光底物（如Ac-DED(Edans)-EEAbuψ[COO]AS-Cys-Lys(Dabcyl)-NH2），通过荧光淬灭变化测定活性。
Q4-是否支持耐药突变体研究？

可提供常见耐药突变（如R155K、A156T）的定制表达服务，需提供突变位点信息。
Q5-该蛋白的储存条件是什么？

溶于含10%甘油的Tris缓冲液（pH 8.0），-80℃保存，避免反复冻融。

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