HCV (HCV-1a) E2 Protein, His Tag_天津卡梅德科技发展有限公司

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HCV (HCV-1a) E2 Protein, His Tag

HCV (HCV-1a) E2 Protein, His Tag

产品编号

KMP4813

别名

丙型肝炎病毒包膜蛋白2, HCV E2, Envelope 2, HCV E2 Protein

规格

50ug
100ug
200ug

DATASHEET MSDS

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产品介绍

Alias	丙型肝炎病毒包膜蛋白2, HCV E2, Envelope 2, HCV E2 Protein
Catalog Number	KMP4813
Product Description	The HCV (HCV-1a) E2 Protein(KMP4813) is produced in HEK293 Cells. A DNA sequence encoding the Hepatitis C virus (serotype 1a, isolate H77) E2 protein (NP_751921.1) (Glu1-Glu278) was expressed with a polyhistidine tag at the C-terminus.
Molecular Name	HCV E2
Species	HCV
Host	HEK293 Cells
Size	50ug, 100ug, 200ug
Purity	>95% as determined by SDS-PAGE
Purification	Affinity purification
Endotoxin	<1.0 EU/ug determined by the LAL method
Formulation	PBS, pH7.4
Background	The influenza viral Hemagglutinin (HA) protein is a homotrimer with a receptor binding pocket on the globular head of each monomer.HA has at least 18 different antigens. These subtypes are named H1 through H18.HA has two functions. Firstly, it allows the recognition of target vertebrate cells, accomplished through the binding to these cells' sialic acid-containing receptors. Secondly, once bound it facilitates the entry of the viral genome into the target cells by causing the fusion of the host endosomal membrane with the viral membrane. The influenza virus Hemagglutinin (HA) protein is translated in cells as a single protein, HA, or hemagglutinin precursor protein. For viral activation, hemagglutinin precursor protein (HA) must be cleaved by a trypsin-like serine endoprotease at a specific site, normally coded for by a single basic amino acid (usually arginine) between the HA1 and HA2 domains of the protein. After cleavage, the two disulfide-bonded protein domains produce the mature form of the protein subunits as a prerequisite for the conformational change necessary for fusion and hence viral infectivity.
Storage Condition	Aliquot and store at -20℃ to -80℃. Avoid repeated freezing and thawing cycles.
Shipping Condition	In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. If supplied as liquid, the proteins will be shipped with dry ice.

FAQ

Q1-E2蛋白在HCV感染中的作用是什么？

E2是包膜糖蛋白，与宿主受体（如CD81、SR-BI）结合，介导病毒进入肝细胞。
Q2-HCV基因1a型有何临床意义？

1a型是北美主要流行亚型，对部分直接抗病毒药物（DAAs）易产生耐药性，研究E2可为药物设计提供靶点。
Q3-His标签是否干扰E2与抗体的结合？

经ELISA验证，标签不影响E2与中和抗体（如AP33）的结合能力。
Q4-能否用于病毒进入抑制实验？

是的，可加入HCVpp（假病毒）系统，评估化合物或抗体对病毒入侵的阻断效果。
Q5-如何提高E2蛋白的溶解度？

表达时添加分子伴侣（如GroEL/ES）或优化缓冲液（含0.5% CHAPS增溶剂）。

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