H1N1 (A/Victoria/4897/2022) HA Trimer Protein, His Tag_天津卡梅德科技发展有限公司

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H1N1 (A/Victoria/4897/2022) HA Trimer Protein, His Tag

H1N1 (A/Victoria/4897/2022) HA Trimer Protein, His Tag

产品编号

KMP4784

别名

甲型流感病毒H1N1血凝素三聚体蛋白, H1N1 HA Trimer, Hemagglutinin trimer Protein, H1N1 HA Trimer Protein

规格

50ug
100ug
200ug

DATASHEET MSDS

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产品介绍

Alias	甲型流感病毒H1N1血凝素三聚体蛋白, H1N1 HA Trimer, Hemagglutinin trimer Protein, H1N1 HA Trimer Protein
Catalog Number	KMP4784
Product Description	The H1N1 (A/Victoria/4897/2022) HA Trimer Protein(KMP4784) is produced in HEK293 Cells. A DNA sequence encoding the Influenza A virus ((A/Victoria/4897/2022) (H1N1)) hemagglutinin (translated amino acids of EPI2397519), termed as HA, was expressed with the bacteriophage T4 fibritin and a polyhistidine tag at the C-terminus.
Molecular Name	H1N1 HA Trimer
Species	H1N1
Host	HEK293 Cells
Size	50ug, 100ug, 200ug
Purity	>95% as determined by SDS-PAGE
Purification	Affinity purification
Endotoxin	<1.0 EU/ug determined by the LAL method
Formulation	20mM Tris, 150mM NaCl, 0.2M Arg, 10% Glycerol, 0.01% Tween20, pH8.5
Background	The influenza viral Hemagglutinin (HA) protein is a homotrimer with a receptor binding pocket on the globular head of each monomer.HA has at least 18 different antigens. These subtypes are named H1 through H18.HA has two functions. Firstly, it allows the recognition of target vertebrate cells, accomplished through the binding to these cells' sialic acid-containing receptors. Secondly, once bound it facilitates the entry of the viral genome into the target cells by causing the fusion of the host endosomal membrane with the viral membrane. The influenza virus Hemagglutinin (HA) protein is translated in cells as a single protein, HA, or hemagglutinin precursor protein. For viral activation, hemagglutinin precursor protein (HA) must be cleaved by a trypsin-like serine endoprotease at a specific site, normally coded for by a single basic amino acid (usually arginine) between the HA1 and HA2 domains of the protein. After cleavage, the two disulfide-bonded protein domains produce the mature form of the protein subunits as a prerequisite for the conformational change necessary for fusion and hence viral infectivity.
Storage Condition	Aliquot and store at -20℃ to -80℃. Avoid repeated freezing and thawing cycles.
Shipping Condition	In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. If supplied as liquid, the proteins will be shipped with dry ice.

FAQ

Q1-维多利亚2022株HA蛋白的公共卫生重要性是什么？

该株是近期季节性H1N1的代表毒株，其HA抗原变异可能影响疫苗有效性，需持续监测。
Q2-三聚体结构对疫苗研发有何意义？

三聚体HA可展示完整抗原表位，诱导更强的中和抗体反应，提升疫苗保护效果。
Q3-该蛋白的表达系统是什么？

采用哺乳动物细胞（Expi293F）表达，保留复杂糖基化修饰，确保免疫原性。
Q4-能否用于快速抗体检测试剂的开发？

是的，三聚体HA可提高ELISA或胶体金试纸条的检测灵敏度和特异性。
Q5-如何避免三聚体解聚？

溶液中添加0.01%聚山梨酯-80（Tween-80），4℃短期保存或-80℃长期保存。

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