H4N2 (A/duck/Hunan/8-19/2009) HA Protein, His Tag_天津卡梅德科技发展有限公司

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H4N2 (A/duck/Hunan/8-19/2009) HA Protein, His Tag

H4N2 (A/duck/Hunan/8-19/2009) HA Protein, His Tag

产品编号

KMP4607

别名

甲型流感病毒H4N2血凝素蛋白, H4N2 HA, Hemagglutinin Protein, H4N2 HA Protein

规格

50ug
100ug
200ug

DATASHEET MSDS

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产品介绍

Alias	甲型流感病毒H4N2血凝素蛋白, H4N2 HA, Hemagglutinin Protein, H4N2 HA Protein
Catalog Number	KMP4607
Product Description	The H4N2 (A/duck/Hunan/8-19/2009) HA Protein(KMP4607) is produced in Insect Cells. A DNA sequence encoding the Influenza A virus (A/duck/Hunan/8-19/2009(H4N2)) hemagglutinin (ADN78261.1) (Met1-Asp527), termed as HA, was expressed with a polyhistidine tag at the C-terminus
Molecular Name	H4N2 HA
Species	H4N2
Host	Insect Cells
Size	50ug, 100ug, 200ug
Purity	>90% as determined by SDS-PAGE
Purification	Affinity purification
Endotoxin	<1.0 EU/ug determined by the LAL method
Formulation	20mM Tris, 500mM NaCl, 10% Glycerol, pH7.0
Background	The influenza viral Hemagglutinin (HA) protein is a homotrimer with a receptor binding pocket on the globular head of each monomer.HA has at least 18 different antigens. These subtypes are named H1 through H18.HA has two functions. Firstly, it allows the recognition of target vertebrate cells, accomplished through the binding to these cells' sialic acid-containing receptors. Secondly, once bound it facilitates the entry of the viral genome into the target cells by causing the fusion of the host endosomal membrane with the viral membrane. The influenza virus Hemagglutinin (HA) protein is translated in cells as a single protein, HA, or hemagglutinin precursor protein. For viral activation, hemagglutinin precursor protein (HA) must be cleaved by a trypsin-like serine endoprotease at a specific site, normally coded for by a single basic amino acid (usually arginine) between the HA1 and HA2 domains of the protein. After cleavage, the two disulfide-bonded protein domains produce the mature form of the protein subunits as a prerequisite for the conformational change necessary for fusion and hence viral infectivity.
Storage Condition	Aliquot and store at -20℃ to -80℃. Avoid repeated freezing and thawing cycles.
Shipping Condition	In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. If supplied as liquid, the proteins will be shipped with dry ice.

FAQ

Q1-H4N2 HA蛋白的宿主（鸭）有何生态学意义？

鸭是禽流感病毒的天然宿主，H4亚型在禽类中广泛传播，研究其HA可监测低致病性病毒向高致病性变异的潜在风险。
Q2-该蛋白是否形成三聚体结构？

经凝胶过滤层析和负染电镜验证，HA蛋白以天然三聚体形式存在，模拟病毒表面构象。
Q3-能否用于疫苗研发？

是的，全长HA蛋白可诱导中和抗体，适用于灭活疫苗或病毒样颗粒（VLP）疫苗设计。
Q4-如何验证其与抗体的结合能力？

通过ELISA或表面等离子共振（SPR）检测与H4特异性多克隆/单克隆抗体的结合亲和力。
Q5-蛋白的内毒素水平是否满足体内实验要求？

内毒素<0.1 EU/μg（LAL法），符合动物免疫实验标准。

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