H10N8 (A/duck/Guangdong/E1/2012) NA Protein, His Tag_天津卡梅德科技发展有限公司

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H10N8 (A/duck/Guangdong/E1/2012) NA Protein, His Tag

H10N8 (A/duck/Guangdong/E1/2012) NA Protein, His Tag

产品编号

KMP4600

别名

甲型流感病毒H10N8神经氨酸酶, H10N8 NA, Neuraminidase Protein, H10N8 NA Protein

规格

50ug
100ug
200ug

DATASHEET MSDS

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产品介绍

Alias	甲型流感病毒H10N8神经氨酸酶, H10N8 NA, Neuraminidase Protein, H10N8 NA Protein
Catalog Number	KMP4600
Product Description	The H10N8 (A/duck/Guangdong/E1/2012) NA Protein(KMP4600) is produced in Insect Cells. A DNA sequence encoding the Influenza A virus (A/duck/Guangdong/E1/2012(H10N8)) Neuraminidase (AFJ19054.1) (Gly38-Gly470) was expressed with a polyhistidine tag at the N-terminus.
Molecular Name	H10N8 NA
Species	H10N8
Host	Insect Cells
Size	50ug, 100ug, 200ug
Purity	>90% as determined by SDS-PAGE
Purification	Affinity purification
Endotoxin	<1.0 EU/ug determined by the LAL method
Formulation	20mM Tris, 500mM NaCl, 10% Glycerol, pH7.4
Background	The influenza viral Hemagglutinin (HA) protein is a homotrimer with a receptor binding pocket on the globular head of each monomer.HA has at least 18 different antigens. These subtypes are named H1 through H18.HA has two functions. Firstly, it allows the recognition of target vertebrate cells, accomplished through the binding to these cells' sialic acid-containing receptors. Secondly, once bound it facilitates the entry of the viral genome into the target cells by causing the fusion of the host endosomal membrane with the viral membrane. The influenza virus Hemagglutinin (HA) protein is translated in cells as a single protein, HA, or hemagglutinin precursor protein. For viral activation, hemagglutinin precursor protein (HA) must be cleaved by a trypsin-like serine endoprotease at a specific site, normally coded for by a single basic amino acid (usually arginine) between the HA1 and HA2 domains of the protein. After cleavage, the two disulfide-bonded protein domains produce the mature form of the protein subunits as a prerequisite for the conformational change necessary for fusion and hence viral infectivity.
Storage Condition	Aliquot and store at -20℃ to -80℃. Avoid repeated freezing and thawing cycles.
Shipping Condition	In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. If supplied as liquid, the proteins will be shipped with dry ice.

FAQ

Q1-NA蛋白在H10N8病毒中的功能重要性是什么？

NA通过切割唾液酸残基促进病毒释放，其活性与病毒传播效率直接相关，是抗病毒药物（如扎那米韦）的靶标。
Q2-该蛋白的酶活性如何检测？

使用荧光底物MUNANA（2’-(4-Methylumbelliferyl)-α-D-N-acetylneuraminic acid），通过荧光强度变化计算酶活单位（NAU）。
Q3-能否用于评估NA抑制剂的耐药性？

是的，可测定药物IC50值，分析突变株（如R292K）对抑制剂的敏感性变化。
Q4-表达系统是否影响NA的活性？

采用昆虫细胞（Sf9）表达系统，保留天然四聚体结构及酶活性，经凝胶过滤验证完整性。
Q5-是否支持大规模药物筛选？

可提供96/384孔板规格的预包被NA蛋白，适用于高通量抑制剂筛选。

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