H4N6 (A/mallard/Ohio/657/2002) NA Protein, His Tag_天津卡梅德科技发展有限公司

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H4N6 (A/mallard/Ohio/657/2002) NA Protein, His Tag

H4N6 (A/mallard/Ohio/657/2002) NA Protein, His Tag

产品编号

KMP4592

别名

甲型流感病毒H4N6神经氨酸酶, H4N6 NA, Neuraminidase Protein, H4N6 NA Protein

规格

50ug
100ug
200ug

DATASHEET MSDS

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产品介绍

Alias	甲型流感病毒H4N6神经氨酸酶, H4N6 NA, Neuraminidase Protein, H4N6 NA Protein
Catalog Number	KMP4592
Product Description	The H4N6 (A/mallard/Ohio/657/2002) NA Protein(KMP4592) is produced in Insect Cells. A DNA sequence encoding the Influenza A virus (A/mallard/Ohio/657/2002(H4N6)) neuraminidase (ABI47998.1) (His36-Lys470) was expressed with a polyhistidine tag at the N-terminus.
Molecular Name	H4N6 NA
Species	H4N6
Host	Insect Cells
Size	50ug, 100ug, 200ug
Purity	>95% as determined by SDS-PAGE
Purification	Affinity purification
Endotoxin	<1.0 EU/ug determined by the LAL method
Formulation	20mM Tris, 500mM NaCl, 10% Glycerol, pH8.0
Background	The influenza viral Hemagglutinin (HA) protein is a homotrimer with a receptor binding pocket on the globular head of each monomer.HA has at least 18 different antigens. These subtypes are named H1 through H18.HA has two functions. Firstly, it allows the recognition of target vertebrate cells, accomplished through the binding to these cells' sialic acid-containing receptors. Secondly, once bound it facilitates the entry of the viral genome into the target cells by causing the fusion of the host endosomal membrane with the viral membrane. The influenza virus Hemagglutinin (HA) protein is translated in cells as a single protein, HA, or hemagglutinin precursor protein. For viral activation, hemagglutinin precursor protein (HA) must be cleaved by a trypsin-like serine endoprotease at a specific site, normally coded for by a single basic amino acid (usually arginine) between the HA1 and HA2 domains of the protein. After cleavage, the two disulfide-bonded protein domains produce the mature form of the protein subunits as a prerequisite for the conformational change necessary for fusion and hence viral infectivity.
Storage Condition	Aliquot and store at -20℃ to -80℃. Avoid repeated freezing and thawing cycles.
Shipping Condition	In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. If supplied as liquid, the proteins will be shipped with dry ice.

FAQ

Q1-H4N6 NA蛋白的宿主（野鸭）有何生态学意义？

野鸭是禽流感病毒的自然宿主，研究其NA蛋白可揭示低致病性病毒在禽类中的传播规律。
Q2-该蛋白是否具有唾液酸酶活性？

是的，活性检测显示其对禽源α-2,3唾液酸受体底物具有高效切割能力。
Q3-能否用于NA抑制剂的耐药性研究？

是的，可评估奥司他韦等药物对NA活性的抑制效率，需配合IC50测定。
Q4-蛋白的糖基化修饰是否完整？

在昆虫细胞（Sf9）中表达，保留哺乳动物类似的N-糖基化修饰。
Q5-是否提供酶动力学参数（Km/Vmax）？

可应要求提供基于MUNANA底物的酶动力学分析报告。

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