H1N1 (A/Brevig Mission/1/1918) Matrix Protein, His Tag_天津卡梅德科技发展有限公司

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H1N1 (A/Brevig Mission/1/1918) Matrix Protein, His Tag

H1N1 (A/Brevig Mission/1/1918) Matrix Protein, His Tag

产品编号

KMP4590

别名

甲型流感病毒H1N1基质蛋白, H1N1 Matrix, VP40, H1N1 Matrix Protein

规格

50ug
100ug
200ug

DATASHEET MSDS

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产品介绍

Alias	甲型流感病毒H1N1基质蛋白, H1N1 Matrix, VP40, H1N1 Matrix Protein
Catalog Number	KMP4590
Product Description	The H1N1 (A/Brevig Mission/1/1918) Matrix Protein(KMP4590) is produced in E. coli. A DNA sequence encoding the influenza A virus (A/Brevig Mission/1/ 1918(H1N1)) matrix protein 1(Q8BAC3.1) (Met1-Lys252), termed as M1, was fused with a N-terminal polyhistidine tag.
Molecular Name	H1N1 Matrix
Species	H1N1
Host	E. coli
Size	50ug, 100ug, 200ug
Purity	>95% as determined by SDS-PAGE
Purification	Affinity purification
Endotoxin	<1.0 EU/ug determined by the LAL method
Formulation	PBS, pH7.4
Background	The influenza viral Hemagglutinin (HA) protein is a homotrimer with a receptor binding pocket on the globular head of each monomer.HA has at least 18 different antigens. These subtypes are named H1 through H18.HA has two functions. Firstly, it allows the recognition of target vertebrate cells, accomplished through the binding to these cells' sialic acid-containing receptors. Secondly, once bound it facilitates the entry of the viral genome into the target cells by causing the fusion of the host endosomal membrane with the viral membrane. The influenza virus Hemagglutinin (HA) protein is translated in cells as a single protein, HA, or hemagglutinin precursor protein. For viral activation, hemagglutinin precursor protein (HA) must be cleaved by a trypsin-like serine endoprotease at a specific site, normally coded for by a single basic amino acid (usually arginine) between the HA1 and HA2 domains of the protein. After cleavage, the two disulfide-bonded protein domains produce the mature form of the protein subunits as a prerequisite for the conformational change necessary for fusion and hence viral infectivity.
Storage Condition	Aliquot and store at -20℃ to -80℃. Avoid repeated freezing and thawing cycles.
Shipping Condition	In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. If supplied as liquid, the proteins will be shipped with dry ice.

FAQ

Q1-Matrix蛋白（M1）的功能是什么？

M1是病毒基质蛋白，介导病毒核衣壳与包膜的连接，并参与病毒出芽和形态维持。
Q2-该毒株的历史意义是什么？

1918年H1N1（西班牙流感）是致死性最强的流感大流行株，研究其M1蛋白有助于解析病毒毒力演化。
Q3-该蛋白能否形成病毒样颗粒（VLPs）？

需与HA/NA共表达，单独M1可自组装成膜结合结构，但无法形成完整VLPs。
Q4-是否适用于电子显微镜研究？

是的，可通过负染电镜观察其寡聚状态，推荐浓度≥0.5 mg/mL。
Q5-如何检测蛋白的正确折叠？

圆二色光谱（CD）分析显示其α-螺旋含量与天然构象一致。

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