H11N6 (A/duck/England/1/1956) HA1 Protein, His Tag_天津卡梅德科技发展有限公司

>>

>>

>>

>

H11N6 (A/duck/England/1/1956) HA1 Protein, His Tag

H11N6 (A/duck/England/1/1956) HA1 Protein, His Tag

产品编号

KMP4573

别名

甲型流感病毒H11N6血凝素蛋白1, H11N6 HA1, Hemagglutinin Protein, H11N6 HA1 Protein

规格

50ug
100ug
200ug

DATASHEET MSDS

立即询价加入询价列表大包装定制

产品介绍

Alias	甲型流感病毒H11N6血凝素蛋白1, H11N6 HA1, Hemagglutinin Protein, H11N6 HA1 Protein
Catalog Number	KMP4573
Molecular Name	H11N6 HA1
Size	50ug, 100ug, 200ug
Purity	>95% as determined by SDS-PAGE
Product Description	The H11N6 (A/duck/England/1/1956) HA1 Protein(KMP4573) is produced in HEK293 Cells. A DNA sequence encoding the Influenza A virus (A/duck/England/1/1956(H11N6)) hemagglutinin (AGB50949.1) (Met1-Arg342), termed as HA1, was expressed with a polyhistidine tag at the C-terminus.
Storage Condition	Aliquot and store at -20℃ to -80℃. Avoid repeated freezing and thawing cycles.
Formulation	PBS, pH7.4
Shipping Condition	In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. If supplied as liquid, the proteins will be shipped with dry ice.
Background	The fourth gene of the EBOV genome encodes a 16-kDa envelope-attached glycoprotein (GP) and a 11 kDa secreted glycoprotein (sGP). Both GP and sGP have an identical 295-residue N-terminus, however, they have different C-terminal sequences. Recently, great attention has been paid to GP for vaccines design and entry inhibitors isolation. GP is a class I fusion protein which assembles as trimers on viral surface and plays an important role in virus entry and attachment. Mature GP is a disulfide-linked heterodimer formed by two subunits, GP1 and GP2, which are generated from the proteolytical process of GP precursor (pre-GP) by cellular furin during virus assembly . The GP1 subunit contains a mucin domain and a receptor-binding domain (RBD), the GP2 subunit has a fusion peptide, a helical heptad-repeat (HR) region, a transmembrane (TM) domain, and a 4-residue cytoplasmic tail. The RBD of GP1 mediates the interaction of EBOV with cellular receptor (e.g. DC-SIGN/LSIGN, TIM-1, hMGL, NPC1, β-integrins, folate receptor-α, and Tyro3 family receptors), of which TIM1 and NPC1 are essential for EBOV entry, the mucin domain having N- and O-linked glycans enhances the viral attachment to cellular hMGL, and participates in shielding key neutralization epitopes, which helps the virus evades immune elimination. There are large conformation changes of GP2 during membrane fusion, which enhance the insertion of fusion loop into cellular membrane and facilitate the release of viral nucleocapsid core to cytoplasm.
Species	H11N6
Host	HEK293 Cells
Purification	Affinity purification
Endotoxin	<1.0 EU/ug determined by the LAL method

FAQ

Q1-1956年分离的H11N6毒株有何特殊性？

该历史毒株为早期H11亚型代表，可用于追溯禽流感病毒的进化及抗原漂移研究。
Q2-该HA1蛋白能否与哺乳动物受体结合？

需通过受体结合实验验证，H11亚型通常偏好禽源α-2,3唾液酸受体。
Q3-是否提供糖基化位点分析数据？

可提供基于质谱的糖基化位点鉴定报告（需额外订购）。
Q4-该蛋白是否适合表面等离子共振（SPR）分析？

是的，低内毒素和高纯度（≥95%）满足SPR芯片固定要求。
Q5-溶解后出现沉淀如何处理？

建议4℃低速离心（10,000×g, 5分钟）后取上清使用。

推荐产品

400-621-6806
614353794
周一至周五 8:30-18:00
0