H10N3 (A/mallard/Minnesota/Sg-00194/2007) HA1 Protein, His Tag_天津卡梅德科技发展有限公司

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H10N3 (A/mallard/Minnesota/Sg-00194/2007) HA1 Protein, His Tag

H10N3 (A/mallard/Minnesota/Sg-00194/2007) HA1 Protein, His Tag

产品编号

KMP4571

别名

甲型流感病毒H10N3血凝素蛋白1, H10N3 HA1, Hemagglutinin Protein, H10N3 HA1 Protein

规格

50ug
100ug
200ug

DATASHEET MSDS

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产品介绍

Alias	甲型流感病毒H10N3血凝素蛋白1, H10N3 HA1, Hemagglutinin Protein, H10N3 HA1 Protein
Catalog Number	KMP4571
Product Description	The H10N3 (A/mallard/Minnesota/Sg-00194/2007) HA1 Protein(KMP4571) is produced in HEK293 Cells. A DNA sequence encoding the Influenza A virus (A/mallard/Minnesota/Sg-00194/2007(H10N3)) hemagglutinin (ACT84107.1) (Met1-Arg340), termed as HA1, was expressed with a C-terminal polyhistidine tag.
Molecular Name	H10N3 HA1
Species	H10N3
Host	HEK293 Cells
Size	50ug, 100ug, 200ug
Purity	>95% as determined by SDS-PAGE
Purification	Affinity purification
Endotoxin	<1.0 EU/ug determined by the LAL method
Formulation	PBS, pH7.4
Background	The fourth gene of the EBOV genome encodes a 16-kDa envelope-attached glycoprotein (GP) and a 11 kDa secreted glycoprotein (sGP). Both GP and sGP have an identical 295-residue N-terminus, however, they have different C-terminal sequences. Recently, great attention has been paid to GP for vaccines design and entry inhibitors isolation. GP is a class I fusion protein which assembles as trimers on viral surface and plays an important role in virus entry and attachment. Mature GP is a disulfide-linked heterodimer formed by two subunits, GP1 and GP2, which are generated from the proteolytical process of GP precursor (pre-GP) by cellular furin during virus assembly . The GP1 subunit contains a mucin domain and a receptor-binding domain (RBD), the GP2 subunit has a fusion peptide, a helical heptad-repeat (HR) region, a transmembrane (TM) domain, and a 4-residue cytoplasmic tail. The RBD of GP1 mediates the interaction of EBOV with cellular receptor (e.g. DC-SIGN/LSIGN, TIM-1, hMGL, NPC1, β-integrins, folate receptor-α, and Tyro3 family receptors), of which TIM1 and NPC1 are essential for EBOV entry, the mucin domain having N- and O-linked glycans enhances the viral attachment to cellular hMGL, and participates in shielding key neutralization epitopes, which helps the virus evades immune elimination. There are large conformation changes of GP2 during membrane fusion, which enhance the insertion of fusion loop into cellular membrane and facilitate the release of viral nucleocapsid core to cytoplasm.
Storage Condition	Aliquot and store at -20℃ to -80℃. Avoid repeated freezing and thawing cycles.
Shipping Condition	In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. If supplied as liquid, the proteins will be shipped with dry ice.

FAQ

Q1-该HA1蛋白的宿主（绿头鸭）有何流行病学意义？

绿头鸭是禽流感病毒的天然宿主，研究其HA1有助于追踪H10亚型在水禽中的进化及潜在人畜共患风险。
Q2-能否用于检测人类感染H10N3的抗体？

是的，可作为ELISA或免疫印迹的抗原，检测血清中H10N3特异性抗体。
Q3-该蛋白的表达系统是什么？

采用HEK293细胞表达系统，确保正确折叠和糖基化修饰。
Q4-是否提供生物安全数据表（SDS）？

是的，所有产品均附详细SDS，包含储存、操作及废弃物处理指南。
Q5-蛋白的推荐工作浓度是多少？

建议根据实验优化，常规ELISA包被浓度为2-10 μg/mL。

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