H9N2 (A/Hong Kong/35820/2009) HA Protein, His Tag_天津卡梅德科技发展有限公司

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H9N2 (A/Hong Kong/35820/2009) HA Protein, His Tag

H9N2 (A/Hong Kong/35820/2009) HA Protein, His Tag

产品编号

KMP4567

别名

甲型流感病毒H9N2血凝素蛋白, H9N2 HA, Hemagglutinin Protein, H9N2 HA Protein

规格

50ug
100ug
200ug

DATASHEET MSDS

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产品介绍

Alias	甲型流感病毒H9N2血凝素蛋白, H9N2 HA, Hemagglutinin Protein, H9N2 HA Protein
Catalog Number	KMP4567
Product Description	The H9N2 (A/Hong Kong/35820/2009) HA Protein(KMP4567) is produced in Insect Cells. A DNA sequence encoding the Influenza A virus (A/Hong Kong/35820/ 2009(H9N2)) hemagglutinin (ADC41853.1) (Met1-Lys523) was expressed with a C-terminal polyhistidine tag.
Molecular Name	H9N2 HA
Species	H9N2
Host	Insect Cells
Size	50ug, 100ug, 200ug
Purity	>85% as determined by SDS-PAGE
Purification	Affinity purification
Endotoxin	<1.0 EU/ug determined by the LAL method
Formulation	20mM Tris, 500mM NaCl, 10% Glycerol, pH7.5
Background	The fourth gene of the EBOV genome encodes a 16-kDa envelope-attached glycoprotein (GP) and a 11 kDa secreted glycoprotein (sGP). Both GP and sGP have an identical 295-residue N-terminus, however, they have different C-terminal sequences. Recently, great attention has been paid to GP for vaccines design and entry inhibitors isolation. GP is a class I fusion protein which assembles as trimers on viral surface and plays an important role in virus entry and attachment. Mature GP is a disulfide-linked heterodimer formed by two subunits, GP1 and GP2, which are generated from the proteolytical process of GP precursor (pre-GP) by cellular furin during virus assembly . The GP1 subunit contains a mucin domain and a receptor-binding domain (RBD), the GP2 subunit has a fusion peptide, a helical heptad-repeat (HR) region, a transmembrane (TM) domain, and a 4-residue cytoplasmic tail. The RBD of GP1 mediates the interaction of EBOV with cellular receptor (e.g. DC-SIGN/LSIGN, TIM-1, hMGL, NPC1, β-integrins, folate receptor-α, and Tyro3 family receptors), of which TIM1 and NPC1 are essential for EBOV entry, the mucin domain having N- and O-linked glycans enhances the viral attachment to cellular hMGL, and participates in shielding key neutralization epitopes, which helps the virus evades immune elimination. There are large conformation changes of GP2 during membrane fusion, which enhance the insertion of fusion loop into cellular membrane and facilitate the release of viral nucleocapsid core to cytoplasm.
Storage Condition	Aliquot and store at -20℃ to -80℃. Avoid repeated freezing and thawing cycles.
Shipping Condition	In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. If supplied as liquid, the proteins will be shipped with dry ice.

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