H7N1 (A/turkey/Italy/4602/99) HA1 Protein, His Tag_天津卡梅德科技发展有限公司

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H7N1 (A/turkey/Italy/4602/99) HA1 Protein, His Tag

H7N1 (A/turkey/Italy/4602/99) HA1 Protein, His Tag

产品编号

KMP4561

别名

甲型流感病毒H7N1血凝素蛋白1, H7N1 HA1, Hemagglutinin Protein, H7N1 HA1 Protein

规格

50ug
100ug
200ug

DATASHEET MSDS

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产品介绍

Alias	甲型流感病毒H7N1血凝素蛋白1, H7N1 HA1, Hemagglutinin Protein, H7N1 HA1 Protein
Catalog Number	KMP4561
Product Description	The H7N1 (A/turkey/Italy/4602/99) HA1 Protein(KMP4561) is produced in HEK293 Cells. A DNA sequence encoding the Influenza A virus (A/turkey/Italy/4602/99(H7N1)) hemagglutinin (CAD38286.1) (Met1-Arg339), termed as HA1, was expressed with a polyhistidine tag at the C-terminus.
Molecular Name	H7N1 HA1
Species	H7N1
Host	HEK293 Cells
Size	50ug, 100ug, 200ug
Purity	>95% as determined by SDS-PAGE
Purification	Affinity purification
Endotoxin	<1.0 EU/ug determined by the LAL method
Formulation	PBS, pH7.4
Background	The fourth gene of the EBOV genome encodes a 16-kDa envelope-attached glycoprotein (GP) and a 11 kDa secreted glycoprotein (sGP). Both GP and sGP have an identical 295-residue N-terminus, however, they have different C-terminal sequences. Recently, great attention has been paid to GP for vaccines design and entry inhibitors isolation. GP is a class I fusion protein which assembles as trimers on viral surface and plays an important role in virus entry and attachment. Mature GP is a disulfide-linked heterodimer formed by two subunits, GP1 and GP2, which are generated from the proteolytical process of GP precursor (pre-GP) by cellular furin during virus assembly . The GP1 subunit contains a mucin domain and a receptor-binding domain (RBD), the GP2 subunit has a fusion peptide, a helical heptad-repeat (HR) region, a transmembrane (TM) domain, and a 4-residue cytoplasmic tail. The RBD of GP1 mediates the interaction of EBOV with cellular receptor (e.g. DC-SIGN/LSIGN, TIM-1, hMGL, NPC1, β-integrins, folate receptor-α, and Tyro3 family receptors), of which TIM1 and NPC1 are essential for EBOV entry, the mucin domain having N- and O-linked glycans enhances the viral attachment to cellular hMGL, and participates in shielding key neutralization epitopes, which helps the virus evades immune elimination. There are large conformation changes of GP2 during membrane fusion, which enhance the insertion of fusion loop into cellular membrane and facilitate the release of viral nucleocapsid core to cytoplasm.
Storage Condition	Aliquot and store at -20℃ to -80℃. Avoid repeated freezing and thawing cycles.
Shipping Condition	In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. If supplied as liquid, the proteins will be shipped with dry ice.

FAQ

Q1-该HA1蛋白是否适用于高致病性禽流感研究？

是的，H7N1（意大利株）为高致病性毒株，需在BSL-3实验室操作活病毒相关实验。
Q2-如何验证蛋白的抗原性？

通过ELISA检测其与康复期火鸡血清的反应性，确认天然表位保留。
Q3-能否用于疫苗佐剂配伍研究？

支持，提供与MF59、铝佐剂或Poly I:C的复合抗原制备及免疫效果评估服务。
Q4-是否提供去内毒素处理？

是的，采用EndoFree纯化柱，确保内毒素水平＜0.05 EU/μg。
Q5-运输和储存条件是什么？

液体需-80℃保存，冻干粉可常温运输，复溶后分装避免反复冻融。

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