H6N8 (A/mallard/Ohio/217/1998) HA1 Protein, His Tag_天津卡梅德科技发展有限公司

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H6N8 (A/mallard/Ohio/217/1998) HA1 Protein, His Tag

H6N8 (A/mallard/Ohio/217/1998) HA1 Protein, His Tag

产品编号

KMP4560

别名

甲型流感病毒H6N8血凝素蛋白1, H6N8 HA1, Hemagglutinin Protein, H6N8 HA1 Protein

规格

50ug
100ug
200ug

DATASHEET MSDS

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产品介绍

Alias	甲型流感病毒H6N8血凝素蛋白1, H6N8 HA1, Hemagglutinin Protein, H6N8 HA1 Protein
Catalog Number	KMP4560
Product Description	The H6N8 (A/mallard/Ohio/217/1998) HA1 Protein(KMP4560) is produced in HEK293 Cells. A DNA sequence encoding the Influenza A virus (A/mallard/Ohio/217/1998(H6N8)) hemagglutinin (ABO52049.1) (Met1-Arg344), termed as HA1, was expressed with a C-terminal polyhistidine tag.
Molecular Name	H6N8 HA1
Species	H6N8
Host	HEK293 Cells
Size	50ug, 100ug, 200ug
Purity	>95% as determined by SDS-PAGE
Purification	Affinity purification
Endotoxin	<1.0 EU/ug determined by the LAL method
Formulation	PBS, pH7.4
Background	The fourth gene of the EBOV genome encodes a 16-kDa envelope-attached glycoprotein (GP) and a 11 kDa secreted glycoprotein (sGP). Both GP and sGP have an identical 295-residue N-terminus, however, they have different C-terminal sequences. Recently, great attention has been paid to GP for vaccines design and entry inhibitors isolation. GP is a class I fusion protein which assembles as trimers on viral surface and plays an important role in virus entry and attachment. Mature GP is a disulfide-linked heterodimer formed by two subunits, GP1 and GP2, which are generated from the proteolytical process of GP precursor (pre-GP) by cellular furin during virus assembly . The GP1 subunit contains a mucin domain and a receptor-binding domain (RBD), the GP2 subunit has a fusion peptide, a helical heptad-repeat (HR) region, a transmembrane (TM) domain, and a 4-residue cytoplasmic tail. The RBD of GP1 mediates the interaction of EBOV with cellular receptor (e.g. DC-SIGN/LSIGN, TIM-1, hMGL, NPC1, β-integrins, folate receptor-α, and Tyro3 family receptors), of which TIM1 and NPC1 are essential for EBOV entry, the mucin domain having N- and O-linked glycans enhances the viral attachment to cellular hMGL, and participates in shielding key neutralization epitopes, which helps the virus evades immune elimination. There are large conformation changes of GP2 during membrane fusion, which enhance the insertion of fusion loop into cellular membrane and facilitate the release of viral nucleocapsid core to cytoplasm.
Storage Condition	Aliquot and store at -20℃ to -80℃. Avoid repeated freezing and thawing cycles.
Shipping Condition	In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. If supplied as liquid, the proteins will be shipped with dry ice.

FAQ

Q1-HA1亚基是否保留血凝活性？

是的，通过血凝试验（HAU）验证，每μg蛋白可凝集≥1×10^6鹅红细胞。
Q2-能否用于受体结合特异性研究？

可以，结合α-2,3或α-2,6唾液酸受体类似物，分析HA1的宿主偏好性。
Q3-如何控制批次间差异？

每批次均通过SDS-PAGE、HPLC和功能活性检测，确保差异≤5%。
Q4-是否提供预包被ELISA板？

支持，提供预包被HA1的96孔板，优化封闭条件，直接用于抗体检测。
Q5-运输过程中如何维持稳定性？

采用干冰运输，附带温度监测设备，建议收货后立即转移至-80℃保存。

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