H6N2 (A/duck/Shantou/83/2000) HA1 Protein, His Tag_天津卡梅德科技发展有限公司

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H6N2 (A/duck/Shantou/83/2000) HA1 Protein, His Tag

H6N2 (A/duck/Shantou/83/2000) HA1 Protein, His Tag

产品编号

KMP4557

别名

甲型流感病毒H6N2血凝素蛋白1, H6N2 HA1, Hemagglutinin Protein, H6N2 HA1 Protein

规格

50ug
100ug
200ug

DATASHEET MSDS

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产品介绍

Alias	甲型流感病毒H6N2血凝素蛋白1, H6N2 HA1, Hemagglutinin Protein, H6N2 HA1 Protein
Catalog Number	KMP4557
Product Description	The H6N2 (A/duck/Shantou/83/2000) HA1 Protein(KMP4557) is produced in HEK293 Cells. A DNA sequence encoding the Influenza A virus (A/duck/Shantou/83/2000(H6N2)) hemagglutinin (ADG44842.1) (Met1-Arg344), termed as HA1, was expressed with a polyhistidine tag at the C-terminus.
Molecular Name	H6N2 HA1
Species	H6N2
Host	HEK293 Cells
Size	50ug, 100ug, 200ug
Purity	>95% as determined by SDS-PAGE
Purification	Affinity purification
Endotoxin	<1.0 EU/ug determined by the LAL method
Formulation	PBS, pH7.4
Background	The fourth gene of the EBOV genome encodes a 16-kDa envelope-attached glycoprotein (GP) and a 11 kDa secreted glycoprotein (sGP). Both GP and sGP have an identical 295-residue N-terminus, however, they have different C-terminal sequences. Recently, great attention has been paid to GP for vaccines design and entry inhibitors isolation. GP is a class I fusion protein which assembles as trimers on viral surface and plays an important role in virus entry and attachment. Mature GP is a disulfide-linked heterodimer formed by two subunits, GP1 and GP2, which are generated from the proteolytical process of GP precursor (pre-GP) by cellular furin during virus assembly . The GP1 subunit contains a mucin domain and a receptor-binding domain (RBD), the GP2 subunit has a fusion peptide, a helical heptad-repeat (HR) region, a transmembrane (TM) domain, and a 4-residue cytoplasmic tail. The RBD of GP1 mediates the interaction of EBOV with cellular receptor (e.g. DC-SIGN/LSIGN, TIM-1, hMGL, NPC1, β-integrins, folate receptor-α, and Tyro3 family receptors), of which TIM1 and NPC1 are essential for EBOV entry, the mucin domain having N- and O-linked glycans enhances the viral attachment to cellular hMGL, and participates in shielding key neutralization epitopes, which helps the virus evades immune elimination. There are large conformation changes of GP2 during membrane fusion, which enhance the insertion of fusion loop into cellular membrane and facilitate the release of viral nucleocapsid core to cytoplasm.
Storage Condition	Aliquot and store at -20℃ to -80℃. Avoid repeated freezing and thawing cycles.
Shipping Condition	In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. If supplied as liquid, the proteins will be shipped with dry ice.

FAQ

Q1-该HA1蛋白是否适用于水禽流感研究？

是的，用于分析鸭类中H6N2的流行趋势及免疫逃逸机制。
Q2-如何验证蛋白的免疫原性？

通过小鼠免疫实验测定抗体效价，并结合中和实验评估功能性抗体水平。
Q3-是否提供去标签蛋白？

支持，通过酶切（如TEV蛋白酶）去除His标签，需在订购时注明。
Q4-能否用于诊断试剂开发？

是的，作为ELISA或胶体金试纸条的包被抗原，检测禽类或环境样本中的病毒抗体。
Q5-运输和储存条件是什么？

液体需-80℃保存，冻干粉可常温运输，复溶后分装避免反复冻融。

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