H5N9 (A/chicken/Italy/22A/1998) HA1 Protein, His Tag_天津卡梅德科技发展有限公司

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H5N9 (A/chicken/Italy/22A/1998) HA1 Protein, His Tag

H5N9 (A/chicken/Italy/22A/1998) HA1 Protein, His Tag

产品编号

KMP4556

别名

甲型流感病毒H5N9血凝素蛋白1, H5N9 HA1, Hemagglutinin Protein, H5N9 HA1 Protein

规格

50ug
100ug
200ug

DATASHEET MSDS

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产品介绍

Alias	甲型流感病毒H5N9血凝素蛋白1, H5N9 HA1, Hemagglutinin Protein, H5N9 HA1 Protein
Catalog Number	KMP4556
Product Description	The H5N9 (A/chicken/Italy/22A/1998) HA1 Protein(KMP4556) is produced in HEK293 Cells. A DNA sequence encoding the Influenza A virus (A/chicken/Italy/22A/ 1998(H5N9)) hemagglutinin (ABR37720.1) (Met1-Arg342) was expressed with a C-terminal polyhistidine tag.
Molecular Name	H5N9 HA1
Species	H5N9
Host	HEK293 Cells
Size	50ug, 100ug, 200ug
Purity	>85% as determined by SDS-PAGE
Purification	Affinity purification
Endotoxin	<1.0 EU/ug determined by the LAL method
Formulation	20mM Tris, 500mM NaCl, 10% Glycerol, pH7.4
Background	The fourth gene of the EBOV genome encodes a 16-kDa envelope-attached glycoprotein (GP) and a 11 kDa secreted glycoprotein (sGP). Both GP and sGP have an identical 295-residue N-terminus, however, they have different C-terminal sequences. Recently, great attention has been paid to GP for vaccines design and entry inhibitors isolation. GP is a class I fusion protein which assembles as trimers on viral surface and plays an important role in virus entry and attachment. Mature GP is a disulfide-linked heterodimer formed by two subunits, GP1 and GP2, which are generated from the proteolytical process of GP precursor (pre-GP) by cellular furin during virus assembly . The GP1 subunit contains a mucin domain and a receptor-binding domain (RBD), the GP2 subunit has a fusion peptide, a helical heptad-repeat (HR) region, a transmembrane (TM) domain, and a 4-residue cytoplasmic tail. The RBD of GP1 mediates the interaction of EBOV with cellular receptor (e.g. DC-SIGN/LSIGN, TIM-1, hMGL, NPC1, β-integrins, folate receptor-α, and Tyro3 family receptors), of which TIM1 and NPC1 are essential for EBOV entry, the mucin domain having N- and O-linked glycans enhances the viral attachment to cellular hMGL, and participates in shielding key neutralization epitopes, which helps the virus evades immune elimination. There are large conformation changes of GP2 during membrane fusion, which enhance the insertion of fusion loop into cellular membrane and facilitate the release of viral nucleocapsid core to cytoplasm.
Storage Condition	Aliquot and store at -20℃ to -80℃. Avoid repeated freezing and thawing cycles.
Shipping Condition	In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. If supplied as liquid, the proteins will be shipped with dry ice.

FAQ

Q1-HA1亚基在疫苗开发中的优势是什么？

HA1聚焦于关键中和表位，可减少非保护性免疫应答，提高疫苗特异性。
Q2-如何检测蛋白的稳定性？

通过动态光散射（DLS）和热稳定性分析（DSF），确认其在储存条件下的均一性。
Q3-能否用于单克隆抗体的表位鉴定？

可以，结合氢氘交换质谱（HDX-MS）或突变扫描，定位抗体结合表位。
Q4-是否支持冻干粉定制？

支持，可添加稳定剂（如海藻糖）优化冻干工艺，延长保存期限。
Q5-运输过程中如何避免降解？

冻干粉可常温运输，液体需干冰运输，附带温度记录仪确保全程低温。

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