H3N2 (A/Babol/36/2005) HA0 Protein_天津卡梅德科技发展有限公司

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H3N2 (A/Babol/36/2005) HA0 Protein

H3N2 (A/Babol/36/2005) HA0 Protein

产品编号

KMP4543

别名

甲型流感病毒H3N2血凝素前体蛋白, H3N2 HA0, Hemagglutinin Protein, H3N2 HA0 Protein

规格

50ug
100ug
200ug

DATASHEET MSDS

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产品介绍

Alias	甲型流感病毒H3N2血凝素前体蛋白, H3N2 HA0, Hemagglutinin Protein, H3N2 HA0 Protein
Catalog Number	KMP4543
Product Description	The H3N2 (A/Babol/36/2005) HA0 Protein(KMP4543) is produced in Insect Cells. A DNA sequence encoding the full length of Influenza A virus (A/Babol/36/2005(H3N2)) hemagglutinin (ACN50256.1) (Gln1-Ile550), termed as HA, was expressed.
Molecular Name	H3N2 HA0
Species	H3N2
Host	Insect Cells
Size	50ug, 100ug, 200ug
Purity	>90% as determined by SDS-PAGE
Purification	Affinity purification
Endotoxin	<0.01 EU/ug determined by the LAL method
Formulation	50mM His, 0.5M NaCl, pH6.2
Background	Papillomaviruses are highly species-specific and can cause squamous epithelial and fibroepithelial tumors in their hosts. Human papillomaviruses (HPVs) are associated with benign and malignant hyperproliferation of cells, with a wide variety of clinical manifestations ranging from condyloma acuminatum to cervical carcinoma. HPV infection is the most common sexually transmitted disease. More than 4 HPV types so far identified are known to infect the genital tract. Genital HPVs are divided into `low risk' HPVs such as HPV 6 and 11 and ‘high risk’ HPV types such as 16, 18, 31, 33, 35, 39, 45 and 52, 58 which are responsible for more than 95% of HPV-induced cervical cancer. Vaccination against these high-risk types seems to be the most feasible prevention for cervical cancer. Indeed, clinical trials have shown prophylactic HPV vaccines to be effective against HPV infection, cervical intraepithelial neoplasia (CIN), and genital warts, but protection is type-specific and the currently developed vaccines target only a few types. These vaccines are based on papillomavirus-like particles (VLPs) composed of the major capsid protein, L1. The L1 protein self assembles into VLPs when expressed at high levels in eukaryotic or insect cells. VLPs are composed of 36 copies of L1 protein organized into 72 pentamers, so-called capsomeres, to form particles that are immunologically indistinguishable from native virions. Experimentally induced VLP antisera are mostly type-specific for neutralization. Minor cross-neutralization has been observed only between closely related HPV types, e.g. HPV6 and 11, HPV18 and 45, or HPV16 and 31. Structure analysis has revealed the presence of several hypervariable loops on the outer surface of the capsid. With a few exceptions, all HPV-neutralizing monoclonal antibodies analyzed so far are type-specific and recognize conformational epitopes within surface-exposed hypervariable loops of the major capsid protein L1.
Storage Condition	Aliquot and store at -20℃ to -80℃. Avoid repeated freezing and thawing cycles.
Shipping Condition	In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. If supplied as liquid, the proteins will be shipped with dry ice.

FAQ

Q1-HA0蛋白与HA1/HA2的区别是什么？

HA0是HA前体蛋白，需宿主蛋白酶切割为HA1和HA2才能激活感染能力，用于研究病毒入侵机制或蛋白酶作用。
Q2-能否用于流感病毒入侵机制研究？

是的，结合TMPRSS2或胰酶处理实验，模拟天然HA活化过程及膜融合活性。
Q3-如何验证HA0的切割效率？

通过SDS-PAGE分析切割后的HA1/HA2比例，并结合红细胞凝集试验验证活性。
Q4-是否支持疫苗研发？

支持，作为灭活疫苗候选抗原，需配合佐剂以增强免疫原性。
Q5-储存条件有何特殊要求？

建议-80℃保存，避免反复冻融，运输时使用干冰并附带温度记录。

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