H1N9 (A/mallard/Ohio/265/1987) HA Protein, His Tag_天津卡梅德科技发展有限公司

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H1N9 (A/mallard/Ohio/265/1987) HA Protein, His Tag

H1N9 (A/mallard/Ohio/265/1987) HA Protein, His Tag

产品编号

KMP4531

别名

甲型流感病毒H1N9血凝素蛋白, H1N9 HA, Hemagglutinin Protein, H1N9 HA Protein

规格

50ug
100ug
200ug

DATASHEET MSDS

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产品介绍

Alias	甲型流感病毒H1N9血凝素蛋白, H1N9 HA, Hemagglutinin Protein, H1N9 HA Protein
Catalog Number	KMP4531
Product Description	The H1N9 (A/mallard/Ohio/265/1987) HA Protein(KMP4531) is produced in Insect Cells. A DNA sequence encoding the Influenza A virus(A/mallard/Ohio/265/1987 (H1N9)) hemagglutinin (ABK40634.1)(Met1-Gln529), termed as HA, was expressed with a polyhistidine tag at the C-terminus.
Molecular Name	H1N9 HA
Species	H1N9
Host	Insect Cells
Size	50ug, 100ug, 200ug
Purity	>90% as determined by SDS-PAGE
Purification	Affinity purification
Endotoxin	<1.0 EU/ug determined by the LAL method
Formulation	20mM Tris, 300mM NaCl, 10% Glycerol, pH8.0
Background	Neuraminidases are enzymes that cleave sialic acid groups from glycoproteins. Influenza neuraminidase is a type of neuraminidase found on the surface of influenza viruses that enables the virus to be released from the host cell. Influenza neuraminidase is composed of four identical subunits arranged in a square. It is normally attached to the virus surface through a long protein stalk. The active sites are in a deep depression on the upper surface. They bind to polysaccharide chains and clip off the sugars at the end. The surface of neuraminidase is decorated with several polysaccharide chains that are similar to the polysaccharide chains that decorate our cell surface proteins. Neuraminidase (NA) and hemagglutinin (HA) are major membrane glycoproteins found on the surface of the influenza virus. Hemagglutinin binds to the sialic acid-containing receptors on the surface of host cells during initial infection and at the end of an infectious cycle. Neuraminidase, on the other hand, cleaves the HA-sialic acid bondage from the newly formed virions and the host cell receptors during budding. Neuraminidase thus is described as a receptor-destroying enzyme that facilitates virus release and efficient spread of the progeny virus from cell to cell. Influenza antibody and influenza antibodies are very important research tools for influenza diagnosis, influenza vaccine development, and anti-influenza virus therapy development. The monoclonal or polyclonal antibody can be raised with protein based antigen or peptide-based antigen. Antibodies raised with protein-based antigen could have better specificity and/or binding affinity than antibodies raised with peptide based antigen, but the cost associated with the recombinant protein antigen is usually higher. Anti-influenza virus hemagglutinin (HA) monoclonal antibody or polyclonal antibody can be used for ELISA assay, western blotting detection, Immunohistochemistry (IHC), flow cytometry, neutralization assay, hemagglutinin inhibition assay, and early diagnosis of influenza viral infection. Sino Biological has developed state-of-the-art monoclonal antibody development technology platforms: mouse monoclonal antibody and rabbit monoclonal antibody. Our rabbit monoclonal antibody platform is one of a kind and offers some unique advantages over mouse monoclonal antibodies, such as high affinity, low cross-reactivity with rabbit polyclonal antibodies.
Storage Condition	Aliquot and store at -20℃ to -80℃. Avoid repeated freezing and thawing cycles.
Shipping Condition	In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. If supplied as liquid, the proteins will be shipped with dry ice.

FAQ

Q1-该HA蛋白是否适用于野鸟流感监测？

是的，作为ELISA抗原检测野鸟血清中的H1N9特异性抗体，支持流行病学调查。
Q2-如何保证蛋白的高纯度？

采用两步纯化（His标签亲和层析+分子筛色谱），纯度≥95%，并提供质检报告。
Q3-能否用于假病毒包装？

可以，与NA蛋白共表达构建假病毒，用于中和实验或抗病毒药物筛选。
Q4-是否提供预包被ELISA板？

支持，提供预包被HA的96孔板，优化封闭条件，直接用于抗体效价检测。
Q5-运输过程中如何维持活性？

采用干冰运输，附带温度监测设备，建议收货后立即转移至-80℃保存。

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