H7N3 (A/turkey/Italy/214845/2002) HA1 Protein, His Tag_天津卡梅德科技发展有限公司

>>

>>

>>

>

H7N3 (A/turkey/Italy/214845/2002) HA1 Protein, His Tag

H7N3 (A/turkey/Italy/214845/2002) HA1 Protein, His Tag

产品编号

KMP4525

别名

甲型流感病毒H7N3血凝素蛋白1, H7N3 HA1, Hemagglutinin Protein, H7N3 HA1 Protein

规格

50ug
100ug
200ug

DATASHEET MSDS

立即询价加入询价列表大包装定制

产品介绍

Alias	甲型流感病毒H7N3血凝素蛋白1, H7N3 HA1, Hemagglutinin Protein, H7N3 HA1 Protein
Catalog Number	KMP4525
Product Description	The H7N3 (A/turkey/Italy/214845/2002) HA1 Protein(KMP4525) is produced in HEK293 Cells. A DNA sequence encoding the Influenza A virus (A/turkey/Italy/214845/2002(H7N3)) hemagglutinin (CAF33017.1) (Met1-Arg349), termed as HA1, was expressed with a C-terminal polyhistidine tag.
Molecular Name	H7N3 HA1
Species	H7N3
Host	HEK293 Cells
Size	50ug, 100ug, 200ug
Purity	>90% as determined by SDS-PAGE
Purification	Affinity purification
Endotoxin	<1.0 EU/ug determined by the LAL method
Formulation	20mM Tris, 300mM NaCl, 10% Glycerol, pH8.0
Background	Neuraminidases are enzymes that cleave sialic acid groups from glycoproteins. Influenza neuraminidase is a type of neuraminidase found on the surface of influenza viruses that enables the virus to be released from the host cell. Influenza neuraminidase is composed of four identical subunits arranged in a square. It is normally attached to the virus surface through a long protein stalk. The active sites are in a deep depression on the upper surface. They bind to polysaccharide chains and clip off the sugars at the end. The surface of neuraminidase is decorated with several polysaccharide chains that are similar to the polysaccharide chains that decorate our cell surface proteins. Neuraminidase (NA) and hemagglutinin (HA) are major membrane glycoproteins found on the surface of the influenza virus. Hemagglutinin binds to the sialic acid-containing receptors on the surface of host cells during initial infection and at the end of an infectious cycle. Neuraminidase, on the other hand, cleaves the HA-sialic acid bondage from the newly formed virions and the host cell receptors during budding. Neuraminidase thus is described as a receptor-destroying enzyme that facilitates virus release and efficient spread of the progeny virus from cell to cell. Influenza antibody and influenza antibodies are very important research tools for influenza diagnosis, influenza vaccine development, and anti-influenza virus therapy development. The monoclonal or polyclonal antibody can be raised with protein based antigen or peptide-based antigen. Antibodies raised with protein-based antigen could have better specificity and/or binding affinity than antibodies raised with peptide based antigen, but the cost associated with the recombinant protein antigen is usually higher. Anti-influenza virus hemagglutinin (HA) monoclonal antibody or polyclonal antibody can be used for ELISA assay, western blotting detection, Immunohistochemistry (IHC), flow cytometry, neutralization assay, hemagglutinin inhibition assay, and early diagnosis of influenza viral infection. Sino Biological has developed state-of-the-art monoclonal antibody development technology platforms: mouse monoclonal antibody and rabbit monoclonal antibody. Our rabbit monoclonal antibody platform is one of a kind and offers some unique advantages over mouse monoclonal antibodies, such as high affinity, low cross-reactivity with rabbit polyclonal antibodies.
Storage Condition	Aliquot and store at -20℃ to -80℃. Avoid repeated freezing and thawing cycles.
Shipping Condition	In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. If supplied as liquid, the proteins will be shipped with dry ice.

FAQ

Q1-HA1亚基与完整HA蛋白的功能有何区别？

HA1包含受体结合域（RBD），负责宿主细胞识别；完整HA需切割为HA1/HA2才能激活膜融合，HA1单独用于表位研究或抗体筛选。
Q2-该蛋白能否用于交叉中和抗体研究？

可以，与其他H7亚型（如H7N7、H7N9）HA1对比，评估抗体对异源毒株的广谱中和能力。
Q3-如何检测HA1的糖基化修饰？

通过PNGase F消化和质谱分析，确认N-糖基化位点与天然病毒一致。
Q4-是否支持定制去糖基化突变体？

支持，可敲除特定糖基化位点（如N165Q），研究其对免疫原性或受体结合的影响。
Q5-储存时是否需要添加稳定剂？

建议添加1% BSA和0.01% Tween-20，防止冻融过程中蛋白聚集或降解。

推荐产品

400-621-6806
614353794
周一至周五 9:00-18:00
0