H7N3 (A/turkey/Italy/214845/2002) HA Protein, His Tag_天津卡梅德科技发展有限公司

>>

>>

>>

>

H7N3 (A/turkey/Italy/214845/2002) HA Protein, His Tag

H7N3 (A/turkey/Italy/214845/2002) HA Protein, His Tag

产品编号

KMP4524

别名

甲型流感病毒H7N3血凝素蛋白, H7N3 HA, Hemagglutinin Protein, H7N3 HA Protein

规格

50ug
100ug
200ug

DATASHEET MSDS

立即询价加入询价列表大包装定制

产品介绍

Alias	甲型流感病毒H7N3血凝素蛋白, H7N3 HA, Hemagglutinin Protein, H7N3 HA Protein
Catalog Number	KMP4524
Product Description	The H7N3 (A/turkey/Italy/214845/2002) HA Protein(KMP4524) is produced in Insect Cells. A DNA sequence encoding the Influenza A virus (A/turkey/Italy/214845/2002(H7N3)) hemagglutinin (CAF33017.1) (Met1-Val524) was expressed with a C-terminal polyhistidine tag.
Molecular Name	H7N3 HA
Species	H7N3
Host	Insect Cells
Size	50ug, 100ug, 200ug
Purity	>90% as determined by SDS-PAGE
Purification	Affinity purification
Endotoxin	<1.0 EU/ug determined by the LAL method
Formulation	20mM Tris, 150mM NaCl, 10% Glycerol, pH7.5
Background	Neuraminidases are enzymes that cleave sialic acid groups from glycoproteins. Influenza neuraminidase is a type of neuraminidase found on the surface of influenza viruses that enables the virus to be released from the host cell. Influenza neuraminidase is composed of four identical subunits arranged in a square. It is normally attached to the virus surface through a long protein stalk. The active sites are in a deep depression on the upper surface. They bind to polysaccharide chains and clip off the sugars at the end. The surface of neuraminidase is decorated with several polysaccharide chains that are similar to the polysaccharide chains that decorate our cell surface proteins. Neuraminidase (NA) and hemagglutinin (HA) are major membrane glycoproteins found on the surface of the influenza virus. Hemagglutinin binds to the sialic acid-containing receptors on the surface of host cells during initial infection and at the end of an infectious cycle. Neuraminidase, on the other hand, cleaves the HA-sialic acid bondage from the newly formed virions and the host cell receptors during budding. Neuraminidase thus is described as a receptor-destroying enzyme that facilitates virus release and efficient spread of the progeny virus from cell to cell. Influenza antibody and influenza antibodies are very important research tools for influenza diagnosis, influenza vaccine development, and anti-influenza virus therapy development. The monoclonal or polyclonal antibody can be raised with protein based antigen or peptide-based antigen. Antibodies raised with protein-based antigen could have better specificity and/or binding affinity than antibodies raised with peptide based antigen, but the cost associated with the recombinant protein antigen is usually higher. Anti-influenza virus hemagglutinin (HA) monoclonal antibody or polyclonal antibody can be used for ELISA assay, western blotting detection, Immunohistochemistry (IHC), flow cytometry, neutralization assay, hemagglutinin inhibition assay, and early diagnosis of influenza viral infection. Sino Biological has developed state-of-the-art monoclonal antibody development technology platforms: mouse monoclonal antibody and rabbit monoclonal antibody. Our rabbit monoclonal antibody platform is one of a kind and offers some unique advantages over mouse monoclonal antibodies, such as high affinity, low cross-reactivity with rabbit polyclonal antibodies.
Storage Condition	Aliquot and store at -20℃ to -80℃. Avoid repeated freezing and thawing cycles.
Shipping Condition	In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. If supplied as liquid, the proteins will be shipped with dry ice.

FAQ

Q1-该HA蛋白在H7N3禽流感病毒中的主要功能是什么？

HA蛋白介导病毒与宿主细胞唾液酸受体的结合及膜融合，是病毒入侵的关键蛋白，也是中和抗体和疫苗研发的核心靶点。
Q2-His标签如何帮助纯化？是否影响蛋白活性？

His标签通过镍柱亲和层析实现高效纯化，位于C端，经血凝试验（HAU）验证不影响受体结合或折叠构象。
Q3-能否用于禽类疫苗开发？

是的，作为灭活疫苗或亚单位疫苗的候选抗原，可评估其对火鸡等高危禽类的免疫保护效果。
Q4-如何验证蛋白的纯度？

通过SDS-PAGE（纯度≥95%）和HPLC分析，并提供质谱鉴定报告，确保无宿主蛋白残留。
Q5-运输条件有何要求？

液体需干冰运输，冻干粉可常温运输，复溶后建议分装保存于-80℃，避免反复冻融。

推荐产品

400-621-6806
614353794
周一至周五 9:00-18:00
0