H2N2 (A/Japan/305/1957) HA1 Protein, His Tag_天津卡梅德科技发展有限公司

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H2N2 (A/Japan/305/1957) HA1 Protein, His Tag

H2N2 (A/Japan/305/1957) HA1 Protein, His Tag

产品编号

KMP4475

别名

甲型流感病毒H2N2血凝素蛋白1, H2N2 HA1, Hemagglutinin Protein, H2N2 HA1 Protein

规格

50ug
100ug
200ug

DATASHEET MSDS

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产品介绍

Alias	甲型流感病毒H2N2血凝素蛋白1, H2N2 HA1, Hemagglutinin Protein, H2N2 HA1 Protein
Catalog Number	KMP4475
Product Description	The H2N2 (A/Japan/305/1957) HA1 Protein(KMP4475) is produced in HEK293 Cells. A DNA sequence encoding the Influenza A virus (A/Japan/305/1957(H2N2)) hemagglutinin (AAO46269.1) (Met1-Glu338), termed as HA1, was expressed with a C-terminal polyhistidine tag.
Molecular Name	H2N2 HA1
Species	H2N2
Host	HEK293 Cells
Size	50ug, 100ug, 200ug
Purity	>90% as determined by SDS-PAGE
Purification	Affinity purification
Endotoxin	<1.0 EU/ug determined by the LAL method
Formulation	PBS, pH7.4
Background	Neuraminidases are enzymes that cleave sialic acid groups from glycoproteins. Influenza neuraminidase is a type of neuraminidase found on the surface of influenza viruses that enables the virus to be released from the host cell. Influenza neuraminidase is composed of four identical subunits arranged in a square. It is normally attached to the virus surface through a long protein stalk. The active sites are in a deep depression on the upper surface. They bind to polysaccharide chains and clip off the sugars at the end. The surface of neuraminidase is decorated with several polysaccharide chains that are similar to the polysaccharide chains that decorate our cell surface proteins. Neuraminidase (NA) and hemagglutinin (HA) are major membrane glycoproteins found on the surface of the influenza virus. Hemagglutinin binds to the sialic acid-containing receptors on the surface of host cells during initial infection and at the end of an infectious cycle. Neuraminidase, on the other hand, cleaves the HA-sialic acid bondage from the newly formed virions and the host cell receptors during budding. Neuraminidase thus is described as a receptor-destroying enzyme that facilitates virus release and efficient spread of the progeny virus from cell to cell. Influenza antibody and influenza antibodies are very important research tools for influenza diagnosis, influenza vaccine development, and anti-influenza virus therapy development. The monoclonal or polyclonal antibody can be raised with protein based antigen or peptide-based antigen. Antibodies raised with protein-based antigen could have better specificity and/or binding affinity than antibodies raised with peptide based antigen, but the cost associated with the recombinant protein antigen is usually higher. Anti-influenza virus hemagglutinin (HA) monoclonal antibody or polyclonal antibody can be used for ELISA assay, western blotting detection, Immunohistochemistry (IHC), flow cytometry, neutralization assay, hemagglutinin inhibition assay, and early diagnosis of influenza viral infection. Sino Biological has developed state-of-the-art monoclonal antibody development technology platforms: mouse monoclonal antibody and rabbit monoclonal antibody. Our rabbit monoclonal antibody platform is one of a kind and offers some unique advantages over mouse monoclonal antibodies, such as high affinity, low cross-reactivity with rabbit polyclonal antibodies.
Storage Condition	Aliquot and store at -20℃ to -80℃. Avoid repeated freezing and thawing cycles.
Shipping Condition	In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. If supplied as liquid, the proteins will be shipped with dry ice.

FAQ

Q1-该HA1蛋白的历史意义是什么？

分离自1957年“亚洲流感”大流行株，研究其抗原变异可为应对未来H2亚型重现提供依据。
Q2-能否用于老年人血清抗体研究？

是的，用于检测老年人群中对H2N2的免疫记忆，评估潜在群体免疫水平。
Q3-是否保留跨物种传播相关突变？

HA1含
Q226L和G228S突变，增强α2,6受体结合能力，提示哺乳动物适应性。
Q4-表达系统是否影响抗原性？

在CHO细胞中表达，糖基化模式接近人源化，确保与临床抗血清的高反应性。
Q5-推荐用于ELISA的包被浓度？

建议0.5-2 μg/ml（碳酸盐缓冲液pH 9.6），4℃过夜包被后封闭使用。

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