DENV (strain New Guinea C) Capsid Protein, His Tag_天津卡梅德科技发展有限公司

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DENV (strain New Guinea C) Capsid Protein, His Tag

DENV (strain New Guinea C) Capsid Protein, His Tag

产品编号

KMP4421

别名

登革热病毒衣壳蛋白, DENV Capsid, CP Protein, DENV Capsid Protein

规格

50ug
100ug
200ug

DATASHEET MSDS

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产品介绍

Alias	登革热病毒衣壳蛋白, DENV Capsid, CP Protein, DENV Capsid Protein
Catalog Number	KMP4421
Product Description	The DENV (Strain New Guinea C) Capsid Protein(KMP4421) is produced in E. coli. A DNA sequence encoding the Capsid protein C [Dengue virus (DENV) (type 2,strain New Guinea C)] (AHF52833.1)(Met1-Arg100) was expressed with a polyhistidine tag at the N-terminus.
Molecular Name	DENV Capsid
Species	DENV
Host	E. coli
Size	50ug, 100ug, 200ug
Purity	>90% as determined by SDS-PAGE
Purification	Affinity purification
Endotoxin	<1.0 EU/ug determined by the LAL method
Formulation	PBS, pH7.4
Background	Envelope of Zika virus is responsible for receptor binding and membrane. Analysis of the envelope protein of Zika, from Brazilian Zika SPH215 (KU321639), indicates predicted B and T cell epitopes in peptides that are consistent with those reported for dengue, YFYF and Japanese encephalitis. The envelope Domain II B cell epitope, to which much dengue non-neutralizing cross-reaction is attributed, is also conserved also in Zika virus, consistent with prior field observations of cross-reactivity with dengue and YF. Domain III of the Zika envelope protein, likely the main specific neutralizing domain, is distinct from recent Brazilian dengue isolates and a recent Peruvian YF isolate (GQ379163), 76% of possible major histocompatibility complex class (MHC) I and MHC II binding peptides and potential B cell linear epitopes are unique to Zika virus.
Storage Condition	Aliquot and store at -20℃ to -80℃. Avoid repeated freezing and thawing cycles.
Shipping Condition	In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. If supplied as liquid, the proteins will be shipped with dry ice.

FAQ

Q1-衣壳蛋白在登革病毒中的功能是什么？

衣壳蛋白包裹病毒RNA形成核衣壳，参与病毒组装和宿主细胞入侵。
Q2-能否用于病毒样颗粒（VLP）构建？

可以，与包膜蛋白共表达形成VLP，模拟天然病毒结构用于疫苗研发。
Q3-如何验证蛋白的稳定性？

通过动态光散射（DLS）和透射电镜（TEM）分析，确认其均一性和颗粒形态。
Q4-是否支持动物免疫实验？

支持，配合佐剂（如弗氏佐剂）诱导高滴度抗体，用于抗血清制备。
Q5-运输和储存条件是什么？

液体需-80℃保存，冻干粉可常温运输，复溶后避免反复冻融。

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