DcCoV (strain HKU23-368F) NP Protein, His Tag_天津卡梅德科技发展有限公司

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DcCoV (strain HKU23-368F) NP Protein, His Tag

DcCoV (strain HKU23-368F) NP Protein, His Tag

产品编号

KMP4420

别名

单峰骆驼冠状病毒核蛋白, DcCoV NP, Nucleoprotein, DcCoV NP Protein

规格

50ug
100ug
200ug

DATASHEET MSDS

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产品介绍

Alias	单峰骆驼冠状病毒核蛋白, DcCoV NP, Nucleoprotein, DcCoV NP Protein
Catalog Number	KMP4420
Product Description	The DcCoV (strain HKU23-368F) NP Protein(KMP4420) is produced in Insect Cells. A DNA sequence encoding the Dromedary camel coronavirus (DcCoV)(strain HKU23-368F) NP (AHN64796.1) (Met1-Ile448) was expressed with a polyhistidine tag at the C-terminus.
Molecular Name	DcCoV NP
Species	DcCoV
Host	Insect Cells
Size	50ug, 100ug, 200ug
Purity	>95% as determined by SDS-PAGE
Purification	Affinity purification
Endotoxin	<1.0 EU/ug determined by the LAL method
Formulation	20mM Tris, 200mM NaCl, 20% Glycerol, pH8.0
Background	Cytomegalovirus (CMV) (human herpesvirus 5) glycoprotein B, also referred as CMV gB or gB, which belongs to the herpesviridae glycoprotein B family. It is a 97-amino acid glycoprotein encoded by the ORF of UL55. Cytomegalovirus Glycoprotein B protein is the most abundant component of the envelope, a target of neutralizing antibodies with at least two defined neutralizing epitopes and an essential replication component. Cytomegalovirus Glycoprotein B protein plays important roles in HCMV entry, cell-cell spread of internal virions, and fusion of infected cells. In addition, Cytomegalovirus Glycoprotein B protein is one envelope protein capable of heparin binding. It forms a physical association with host cell annexin II independent of the presence of calcium.
Storage Condition	Aliquot and store at -20℃ to -80℃. Avoid repeated freezing and thawing cycles.
Shipping Condition	In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. If supplied as liquid, the proteins will be shipped with dry ice.

FAQ

Q1-NP蛋白在冠状病毒中的功能是什么？

核衣壳蛋白（NP）包裹病毒RNA，参与基因组包装和转录，是诊断标志物和疫苗靶点。
Q2-能否用于RT-PCR检测的阳性对照？

可以，作为RNA提取和扩增的质控品，或用于建立核酸检测标准曲线。
Q3-如何避免核酸污染？

采用无核酸酶的表达系统，并通过DNase/RNase处理确保无残留病毒RNA/DNA。
Q4-是否支持诊断试剂的开发？

支持，作为ELISA或免疫层析试剂的捕获抗原，检测患者血清中的IgG/IgM抗体。
Q5-运输条件有何要求？

液体需-80℃保存，冻干粉可常温运输，复溶后分装避免反复冻融。

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