ZIKV (strain Zika SPH2016) Envelope GP E Protein, Fc Tag
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产品编号
KMP4919
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别名
寨卡病毒包膜蛋白E, ZIKV Envelope GP E, Envelope, ZIKV Envelope GP E Protein
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规格
- 50ug
- 100ug
- 200ug
产品介绍
| Catalog Number | KMP4919 |
| Alias | 寨卡病毒包膜蛋白E, ZIKV Envelope GP E, Envelope, ZIKV Envelope GP E Protein |
| Size | 50ug, 100ug, 200ug |
| Product Description | The ZIKV (strain Zika SPH2016) Envelope GP E Protein(KMP4919) is produced in HEK293 Cells. A DNA sequence encoding the Zika virus (strain Zika SPH2016) E_stem (Stem/anchor domain of flavivirus envelope glycoprotein E) (ALU33341.1) (Gly698-Ala794) was expressed with the Fc region of human IgG1 at the C-terminus. |
| Molecular Name | ZIKV Envelope GP E |
| Species | ZIKV |
| Host | HEK293 Cells |
| Purity | >90% as determined by SDS-PAGE |
| Purification | Affinity purification |
| Storage Condition | Aliquot and store at -20℃ to -80℃. Avoid repeated freezing and thawing cycles. |
| Formulation | 20mM Tris, 500mM NaCl, 10% Glycerol, pH8.0 |
| Shipping Condition | In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. If supplied as liquid, the proteins will be shipped with dry ice. |
| Endotoxin | <1.0 EU/ug determined by the LAL method |
FAQ
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Q1-GP E蛋白的命名与功能有何关联？
GP E即包膜糖蛋白（Envelope Glycoprotein），与M蛋白共同形成病毒表面刺突，介导宿主细胞入侵。
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Q2-2016株与2015株的差异是什么？
2016株可能包含抗原漂移突变，需测序比对确认，但其GP E核心功能（受体结合）高度保守。
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Q3-能否用于快速诊断试纸条开发？
是的，作为抗原可检测患者血清中的IgM/IgG，但需与登革病毒抗原区分以避免交叉反应。
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Q4-如何验证糖基化修饰？
质谱鉴定N-糖基化位点（如Asn-154），或PNGase F酶切后观察SDS-PAGE迁移率变化。
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Q5-运输过程中如何维持活性？
干冰运输，到货后立即-80℃保存；避免高温暴露导致蛋白变性。
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