SARS (isolate Tor2) Spike RBD Protein, His Tag, Avi Tag
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产品编号
KMP4908
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别名
冠状病毒刺突蛋白受体结构域, SARS Spike RBD, S Protein, SARS Spike RBD Protein
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规格
- 50ug
- 100ug
- 200ug
产品介绍
| Catalog Number | KMP4908 |
| Alias | 冠状病毒刺突蛋白受体结构域, SARS Spike RBD, S Protein, SARS Spike RBD Protein |
| Size | 50ug, 100ug, 200ug |
| Product Description | The SARS (Isolate Tor2) Spike RBD Protein(KMP4908) is produced in HEK293 Cells. A DNA sequence encoding the SARS-CoV (isolate Tor2) spike RBD (NP_828851.1) (Arg306-Phe527) was expressed with a c-terminal AVI tagged polyhistidine tag at the C-terminus. The expressed protein was biotinylated in vivo by the Biotin-Protein ligase (BirA enzyme) which is co-expressed. |
| Molecular Name | SARS Spike RBD |
| Species | SARS |
| Host | HEK293 Cells |
| Purity | >90% as determined by SDS-PAGE |
| Purification | Affinity purification |
| Storage Condition | Aliquot and store at -20℃ to -80℃. Avoid repeated freezing and thawing cycles. |
| Formulation | 50mM Tris, 250mM NaCl, 1mM EDTA, 50% Glycerol, pH7.0 |
| Shipping Condition | In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. If supplied as liquid, the proteins will be shipped with dry ice. |
| Endotoxin | <1.0 EU/ug determined by the LAL method |
FAQ
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Q1-RBD蛋白的功能和意义是什么？
RBD（受体结合域）是SARS-CoV刺突蛋白的关键区域，直接结合宿主ACE2受体，介导病毒入侵，是中和抗体和疫苗研发的核心靶点。
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Q2-His和Avi双标签的作用是什么？
His标签用于镍柱纯化，Avi标签允许位点特异性生物素化，便于基于链霉亲和素的检测（如ELISA、SPR或单颗粒分析）。
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Q3-如何验证RBD与ACE2的结合活性？
通过表面等离子共振（SPR）或ELISA检测其与重组ACE2蛋白的亲和力（KD值通常为nM级）。
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Q4-能否用于mRNA疫苗抗原设计？
是的，RBD是mRNA疫苗（如Moderna早期候选疫苗）的常用抗原，可诱导高效中和抗体。
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Q5-储存条件如何优化？
-80℃保存，避免反复冻融；生物素化后4℃稳定1周，长期需添加50%甘油防冻。
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