MERS-CoV S1 Protein, His Tag_天津卡梅德科技发展有限公司

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MERS-CoV S1 Protein, His Tag

MERS-CoV S1 Protein, His Tag

产品编号

KMP4887

别名

中东呼吸综合症冠状病毒刺突蛋白S1, MERS-CoV S1, Spike1 Protein, MERS-CoV S1 Protein

规格

50ug
100ug
200ug

DATASHEET MSDS

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产品介绍

Alias	中东呼吸综合症冠状病毒刺突蛋白S1, MERS-CoV S1, Spike1 Protein, MERS-CoV S1 Protein
Catalog Number	KMP4887
Molecular Name	MERS-CoV S1
Size	50ug, 100ug, 200ug
Purity	>95% as determined by SDS-PAGE
Product Description	The MERS-CoV S1 Protein(KMP4887) is produced in HEK293 Cells. A DNA sequence encoding the spike protein S1 Subunit MERS-CoV (AFS88936.1)(Met1-Glu725) was fused with a polyhistidine tag at the C-terminus.
Storage Condition	Aliquot and store at -20℃ to -80℃. Avoid repeated freezing and thawing cycles.
Formulation	20mM Tris, 300mM NaCl, 10% Glycerol, pH8.5
Shipping Condition	In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. If supplied as liquid, the proteins will be shipped with dry ice.
Species	MERS-CoV
Host	HEK293 Cells
Purification	Affinity purification
Endotoxin	<1.0 EU/ug determined by the LAL method

FAQ

Q1-S1蛋白在MERS-CoV感染中的作用是什么？

S1是刺突蛋白（Spike）的受体结合亚基，识别宿主细胞表面DPP4受体，介导病毒入侵呼吸道和肠道上皮细胞。
Q2-His标签是否影响S1的受体结合活性？

标签位于C末端，经表面等离子共振（SPR）验证，不影响与DPP4的亲和力（KD值约为10^-6 M）。
Q3-该蛋白能否用于疫苗研发？

是的，S1可诱导中和抗体，是亚单位疫苗或mRNA疫苗设计的核心抗原。
Q4-如何验证蛋白的天然构象？

通过圆二色光谱（CD）和负染电镜分析，确认其保留β-折叠为主的天然二级结构。
Q5-储存条件有何建议？

-80℃保存，避免反复冻融；溶解后4℃稳定1周，长期使用需分装冻存。

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