Influenza B (B/Brisbane/60/2008) NA Protein_天津卡梅德科技发展有限公司

>>

>>

>>

>

Influenza B (B/Brisbane/60/2008) NA Protein

Influenza B (B/Brisbane/60/2008) NA Protein

产品编号

KMP4860

别名

乙型流感病毒神经氨酸酶, Influenza B NA, Neuraminidase Protein, Influenza B NA Protein

规格

50ug
100ug
200ug

DATASHEET MSDS

立即询价加入询价列表大包装定制

产品介绍

Alias	乙型流感病毒神经氨酸酶, Influenza B NA, Neuraminidase Protein, Influenza B NA Protein
Catalog Number	KMP4860
Product Description	The Influenza B (B/Brisbane/60/2008) NA Protein(KMP4860) is produced in HEK293 Cells. A DNA sequence encoding Influenza B (B/Brisbane/60/2008) Neuraminidase (4CPL_A) (Met1-Leu466), termed as NA, was expressed.
Molecular Name	Influenza B NA
Species	Influenza B
Host	HEK293 Cells
Size	50ug, 100ug, 200ug
Purity	>95% as determined by SDS-PAGE
Purification	Affinity purification
Endotoxin	<1.0 EU/ug determined by the LAL method
Formulation	PBS, pH7.4
Background	The spike (S) glycoprotein of coronaviruses contains protrusions that will only bind to certain receptors on the host cell. Known receptors bind S1 are ACE2, angiotensin-converting enzyme 2, DPP4, dipeptidyl peptidase-4, APN, aminopeptidase N, CEACAM, carcinoembryonic antigen-related cell adhesion molecule 1, Sia, sialic acid, O-ac Sia, O-acetylated sialic acid. The spike is essential for both host specificity and viral infectivity. The term 'peplomer' is typically used to refer to a grouping of heterologous proteins on the virus surface that function together. The spike (S) glycoprotein of coronaviruses is known to be essential in the binding of the virus to the host cell at the advent of the infection process. It's been reported that SARS-CoV-2 (COVID-19 coronavirus, 2019-nCoV) can infect the human respiratory epithelial cells through interaction with the human ACE2 receptor. The spike protein is a large type I transmembrane protein containing two subunits, S1 and S2. S1 mainly contains a receptor binding domain (RBD), which is responsible for recognizing the cell surface receptor. S2 contains basic elements needed for the membrane fusion. The S protein plays key parts in the induction of neutralizing-antibody and T-cell responses, as well as protective immunity. The main functions for the Spike protein are summarized as: Mediate receptor binding and membrane fusion, Defines the range of the hosts and specificity of the virus, Main component to bind with the neutralizing antibody, Key target for vaccine design, Can be transmitted between different hosts through gene recombination or mutation of the receptor binding domain (RBD), leading to a higher mortality rate.
Storage Condition	Aliquot and store at -20℃ to -80℃. Avoid repeated freezing and thawing cycles.
Shipping Condition	In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. If supplied as liquid, the proteins will be shipped with dry ice.

FAQ

Q1-NA蛋白的功能是什么？

神经氨酸酶（NA）切割宿主细胞表面唾液酸残基，促进病毒释放，是奥司他韦等药物的靶点。
Q2-该毒株的疫苗相关性如何？

B/Brisbane/60/2008是WHO曾推荐的疫苗株，其NA蛋白用于疫苗效力评估及药物耐药性监测。
Q3-如何检测NA酶活性？

使用荧光底物MUNANA（检测荧光强度变化）或比色法（如2,3-二氧苯甲酸法）。
Q4-未添加His标签如何纯化？

通过离子交换层析或亲和层析（如小麦胚凝集素WGA结合唾液酸类似物）。
Q5-能否用于高通量药物筛选？

是的，适用于96孔板格式的酶活抑制实验，评估化合物IC50值。

推荐产品

400-621-6806
614353794
周一至周五 9:00-18:00
0