Influenza B (B/Yamagata/16/1988) HA1 Protein, His Tag_天津卡梅德科技发展有限公司

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Influenza B (B/Yamagata/16/1988) HA1 Protein, His Tag

Influenza B (B/Yamagata/16/1988) HA1 Protein, His Tag

产品编号

KMP4855

别名

乙型流感病毒血凝素蛋白1, Influenza B HA1, Hemagglutinin Protein, Influenza B HA1 Protein

规格

50ug
100ug
200ug

DATASHEET MSDS

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产品介绍

Alias	乙型流感病毒血凝素蛋白1, Influenza B HA1, Hemagglutinin Protein, Influenza B HA1 Protein
Catalog Number	KMP4855
Product Description	The Influenza B (B/Yamagata/16/1988) HA1 Protein(KMP4855) is produced in HEK293 Cells. A DNA sequence encoding the Influenza B virus (B/Yamagata/16/1988) hemagglutinin (Met1-Arg360), termed as HA1, was expressed with a polyhistidine tag at the C-terminus.
Molecular Name	Influenza B HA1
Species	Influenza B
Host	HEK293 Cells
Size	50ug, 100ug, 200ug
Purity	>85% as determined by SDS-PAGE
Purification	Affinity purification
Endotoxin	<1.0 EU/ug determined by the LAL method
Formulation	PBS, 300mM NaCl, pH7.4
Background	The spike (S) glycoprotein of coronaviruses contains protrusions that will only bind to certain receptors on the host cell. Known receptors bind S1 are ACE2, angiotensin-converting enzyme 2, DPP4, dipeptidyl peptidase-4, APN, aminopeptidase N, CEACAM, carcinoembryonic antigen-related cell adhesion molecule 1, Sia, sialic acid, O-ac Sia, O-acetylated sialic acid. The spike is essential for both host specificity and viral infectivity. The term 'peplomer' is typically used to refer to a grouping of heterologous proteins on the virus surface that function together. The spike (S) glycoprotein of coronaviruses is known to be essential in the binding of the virus to the host cell at the advent of the infection process. It's been reported that SARS-CoV-2 (COVID-19 coronavirus, 2019-nCoV) can infect the human respiratory epithelial cells through interaction with the human ACE2 receptor. The spike protein is a large type I transmembrane protein containing two subunits, S1 and S2. S1 mainly contains a receptor binding domain (RBD), which is responsible for recognizing the cell surface receptor. S2 contains basic elements needed for the membrane fusion. The S protein plays key parts in the induction of neutralizing-antibody and T-cell responses, as well as protective immunity. The main functions for the Spike protein are summarized as: Mediate receptor binding and membrane fusion, Defines the range of the hosts and specificity of the virus, Main component to bind with the neutralizing antibody, Key target for vaccine design, Can be transmitted between different hosts through gene recombination or mutation of the receptor binding domain (RBD), leading to a higher mortality rate.
Storage Condition	Aliquot and store at -20℃ to -80℃. Avoid repeated freezing and thawing cycles.
Shipping Condition	In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. If supplied as liquid, the proteins will be shipped with dry ice.

FAQ

Q1-HA1蛋白在乙型流感病毒中的作用是什么？

HA1是血凝素（HA）的受体结合亚基，识别宿主唾液酸受体（α-2,6偏好），介导病毒入侵。
Q2-Yamagata/1988株的流行病学意义是什么？

该株是乙型流感Yamagata谱系的早期代表株，研究其HA1可追溯抗原漂移及疫苗株演化。
Q3-如何验证HA1的受体结合活性？

通过红细胞凝集实验（HA Assay）或表面等离子共振（SPR）检测与唾液酸受体的结合。
Q4-是否适用于单克隆抗体制备？

是的，免疫动物后筛选HA1特异性中和抗体（如针对受体结合域表位）。
Q5-蛋白的糖基化修饰是否保留？

哺乳动物细胞（如HEK293）表达保留天然糖基化，昆虫细胞表达可能简化糖链结构。

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