Influenza B (B/Malaysia/2506/2004) HA Protein, His Tag_天津卡梅德科技发展有限公司

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Influenza B (B/Malaysia/2506/2004) HA Protein, His Tag

Influenza B (B/Malaysia/2506/2004) HA Protein, His Tag

产品编号

KMP4853

别名

乙型流感病毒血凝素蛋白, Influenza B HA, Hemagglutinin Protein, Influenza B HA Protein

规格

50ug
100ug
200ug

DATASHEET MSDS

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产品介绍

Alias	乙型流感病毒血凝素蛋白, Influenza B HA, Hemagglutinin Protein, Influenza B HA Protein
Catalog Number	KMP4853
Product Description	The Influenza B (B/Malaysia/2506/2004) HA Protein(KMP4853) is produced in Insect Cells. A DNA sequence encoding the influenza B virus hemagglutinin (B/Malaysia/2506/2004) (ACO05957.1) (Met 1-Ala 556), termed as HA (HA1+HA2,uncleaved), was fused with a C-terminal polyhistidine tag.
Molecular Name	Influenza B HA
Species	Influenza B
Host	Insect Cells
Size	50ug, 100ug, 200ug
Purity	>90% as determined by SDS-PAGE
Purification	Affinity purification
Endotoxin	<1.0 EU/ug determined by the LAL method
Formulation	20mM Tris, 300mM NaCl, 10% Glycerol, pH7.0
Background	The spike (S) glycoprotein of coronaviruses contains protrusions that will only bind to certain receptors on the host cell. Known receptors bind S1 are ACE2, angiotensin-converting enzyme 2, DPP4, dipeptidyl peptidase-4, APN, aminopeptidase N, CEACAM, carcinoembryonic antigen-related cell adhesion molecule 1, Sia, sialic acid, O-ac Sia, O-acetylated sialic acid. The spike is essential for both host specificity and viral infectivity. The term 'peplomer' is typically used to refer to a grouping of heterologous proteins on the virus surface that function together. The spike (S) glycoprotein of coronaviruses is known to be essential in the binding of the virus to the host cell at the advent of the infection process. It's been reported that SARS-CoV-2 (COVID-19 coronavirus, 2019-nCoV) can infect the human respiratory epithelial cells through interaction with the human ACE2 receptor. The spike protein is a large type I transmembrane protein containing two subunits, S1 and S2. S1 mainly contains a receptor binding domain (RBD), which is responsible for recognizing the cell surface receptor. S2 contains basic elements needed for the membrane fusion. The S protein plays key parts in the induction of neutralizing-antibody and T-cell responses, as well as protective immunity. The main functions for the Spike protein are summarized as: Mediate receptor binding and membrane fusion, Defines the range of the hosts and specificity of the virus, Main component to bind with the neutralizing antibody, Key target for vaccine design, Can be transmitted between different hosts through gene recombination or mutation of the receptor binding domain (RBD), leading to a higher mortality rate.
Storage Condition	Aliquot and store at -20℃ to -80℃. Avoid repeated freezing and thawing cycles.
Shipping Condition	In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. If supplied as liquid, the proteins will be shipped with dry ice.

FAQ

Q1-乙型流感HA蛋白的功能是什么？

HA介导病毒结合宿主唾液酸受体（α-2,6偏好），并触发膜融合，是疫苗主要抗原。
Q2-该毒株的疫苗匹配性如何？

B/Malaysia/2506/2004是WHO推荐的疫苗株之一，其HA蛋白用于疫苗生产及效力评估。
Q3-如何验证HA的受体结合活性？

通过红细胞凝集实验（HA Assay）或糖芯片技术检测唾液酸受体亲和力。
Q4-是否形成三聚体？

经凝胶过滤层析验证，以天然三聚体形式存在，保留免疫原性。
Q5-运输过程中如何维持活性？

干冰运输，到货后立即-80℃保存，避免温度波动导致变性。

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