HSV2 (strain 333) gD Protein(ECD), His Tag_天津卡梅德科技发展有限公司

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HSV2 (strain 333) gD Protein(ECD), His Tag

HSV2 (strain 333) gD Protein(ECD), His Tag

产品编号

KMP4835

别名

单纯疱疹病毒包膜糖蛋白D, HSV2 gD, Glycoprotein D, HSV2 gD Protein

规格

50ug
100ug
200ug

DATASHEET MSDS

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产品介绍

Alias	单纯疱疹病毒包膜糖蛋白D, HSV2 gD, Glycoprotein D, HSV2 gD Protein
Catalog Number	KMP4835
Product Description	The HSV2 (strain 333) gD Protein(KMP4835) is produced in Insect Cells. A DNA sequence encoding the HSV 2 (strain 333 (HSV2)) gD (translated amino acids of P03172) (Lys26-Pro339) was expressed with a polyhistidine tag at the C-terminus.
Molecular Name	HSV2 gD
Species	HSV2
Host	Insect Cells
Size	50ug, 100ug, 200ug
Purity	>95% as determined by SDS-PAGE
Purification	Affinity purification
Endotoxin	<1.0 EU/ug determined by the LAL method
Formulation	PBS, pH7.4
Background	The influenza viral Hemagglutinin (HA) protein is a homotrimer with a receptor binding pocket on the globular head of each monomer.HA has at least 18 different antigens. These subtypes are named H1 through H18.HA has two functions. Firstly, it allows the recognition of target vertebrate cells, accomplished through the binding to these cells' sialic acid-containing receptors. Secondly, once bound it facilitates the entry of the viral genome into the target cells by causing the fusion of the host endosomal membrane with the viral membrane. The influenza virus Hemagglutinin (HA) protein is translated in cells as a single protein, HA, or hemagglutinin precursor protein. For viral activation, hemagglutinin precursor protein (HA) must be cleaved by a trypsin-like serine endoprotease at a specific site, normally coded for by a single basic amino acid (usually arginine) between the HA1 and HA2 domains of the protein. After cleavage, the two disulfide-bonded protein domains produce the mature form of the protein subunits as a prerequisite for the conformational change necessary for fusion and hence viral infectivity.
Storage Condition	Aliquot and store at -20℃ to -80℃. Avoid repeated freezing and thawing cycles.
Shipping Condition	In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. If supplied as liquid, the proteins will be shipped with dry ice.

FAQ

Q1-gD蛋白的功能是什么？

gD（糖蛋白D）是HSV-2进入宿主细胞的关键蛋白，结合HVEM或nectin-1受体触发膜融合。
Q2-该蛋白能否用于治疗性抗体制备？

是的，免疫动物后可筛选中和抗体（如mAb HD1），用于被动免疫治疗。
Q3-ECD（胞外域）是否保留天然构象？

经圆二色光谱（CD）验证，其α-螺旋和β-折叠含量与天然蛋白一致。
Q4-是否适用于假病毒中和实验？

是的，与HSV-1 gB/gH/gL共表达可生成嵌合假病毒，评估抗体效价。
Q5-储存条件有何建议？

溶于含5%甘油的PBS，-80℃保存；避免反复冻融，分装后使用。

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