HCoV-NL63 Nucleocapsid Protein, His Tag_天津卡梅德科技发展有限公司

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HCoV-NL63 Nucleocapsid Protein, His Tag

HCoV-NL63 Nucleocapsid Protein, His Tag

产品编号

KMP4831

别名

人类冠状病毒核衣壳蛋白, HCoV-NL63 Nucleocapsid, N Protein, HCoV-NL63 Nucleocapsid Protein

规格

50ug
100ug
200ug

DATASHEET MSDS

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产品介绍

Alias	人类冠状病毒核衣壳蛋白, HCoV-NL63 Nucleocapsid, N Protein, HCoV-NL63 Nucleocapsid Protein
Catalog Number	KMP4831
Product Description	The HCoV-NL63 (HCoV-NL63) Nucleocapsid Protein(KMP4831) is produced in E. coli. A DNA sequence encoding the Human coronavirus (HCoV-NL63) Nucleocapsid (YP_003771.1) (Met1-His377) was expressed with a polyhistide tag at the N-terminus.
Molecular Name	HCoV-NL63 Nucleocapsid
Species	HCoV-NL63
Host	E. coli
Size	50ug, 100ug, 200ug
Purity	>90% as determined by SDS-PAGE
Purification	Affinity purification
Endotoxin	<1.0 EU/ug determined by the LAL method
Formulation	20mM Tris, 150mM NaCl, 10% Glycerol, pH7.5
Background	The influenza viral Hemagglutinin (HA) protein is a homotrimer with a receptor binding pocket on the globular head of each monomer.HA has at least 18 different antigens. These subtypes are named H1 through H18.HA has two functions. Firstly, it allows the recognition of target vertebrate cells, accomplished through the binding to these cells' sialic acid-containing receptors. Secondly, once bound it facilitates the entry of the viral genome into the target cells by causing the fusion of the host endosomal membrane with the viral membrane. The influenza virus Hemagglutinin (HA) protein is translated in cells as a single protein, HA, or hemagglutinin precursor protein. For viral activation, hemagglutinin precursor protein (HA) must be cleaved by a trypsin-like serine endoprotease at a specific site, normally coded for by a single basic amino acid (usually arginine) between the HA1 and HA2 domains of the protein. After cleavage, the two disulfide-bonded protein domains produce the mature form of the protein subunits as a prerequisite for the conformational change necessary for fusion and hence viral infectivity.
Storage Condition	Aliquot and store at -20℃ to -80℃. Avoid repeated freezing and thawing cycles.
Shipping Condition	In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. If supplied as liquid, the proteins will be shipped with dry ice.

FAQ

Q1-核衣壳蛋白（NP）的功能是什么？

NP包裹病毒RNA形成核糖核蛋白复合体，参与基因组包装和宿主免疫逃逸（如抑制干扰素信号）。
Q2-能否用于COVID-19诊断试剂的交叉验证？

否，NL63 NP与SARS-CoV-2 NP无交叉反应，但可用于特异性检测NL63感染。
Q3-如何检测NP的RNA结合能力？

使用荧光标记的RNA探针，通过荧光偏振或电泳迁移率变动分析（EMSA）验证结合活性。
Q4-蛋白的纯度如何？

经镍柱亲和层析和分子筛纯化，SDS-PAGE验证纯度≥95%。
Q5-是否支持动物免疫实验？

是的，已用于小鼠免疫产生高滴度抗体，推荐弗氏佐剂配伍。

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