HCoV-OC43 Spike Protein(ECD), His Tag_天津卡梅德科技发展有限公司

>>

>>

>>

>

HCoV-OC43 Spike Protein(ECD), His Tag

HCoV-OC43 Spike Protein(ECD), His Tag

产品编号

KMP4829

别名

人类冠状病毒刺突蛋白, HCoV-OC43 Spike, S Protein, HCoV-OC43 Spike Protein

规格

50ug
100ug
200ug

DATASHEET MSDS

立即询价加入询价列表大包装定制

产品介绍

Alias	人类冠状病毒刺突蛋白, HCoV-OC43 Spike, S Protein, HCoV-OC43 Spike Protein
Catalog Number	KMP4829
Product Description	The HCoV-OC43 (HCoV-OC43) Spike Protein(KMP4829) is produced in Insect Cells. A DNA sequence encoding the human coronavirus (HCoV-OC43) spike protein (S1+S2 ECD) (AVR40344.1) (Met1-Pro1304) was expressed with a polyhistidine tag at the C-terminus. The purified protein was biotinylated in vitro.
Molecular Name	HCoV-OC43 Spike
Species	HCoV-OC43
Host	Insect Cells
Size	50ug, 100ug, 200ug
Purity	>95% as determined by SDS-PAGE
Purification	Affinity purification
Endotoxin	<1.0 EU/ug determined by the LAL method
Formulation	20mM PB, 300mM NaCl, 10% Glycerol, pH7.0
Background	The influenza viral Hemagglutinin (HA) protein is a homotrimer with a receptor binding pocket on the globular head of each monomer.HA has at least 18 different antigens. These subtypes are named H1 through H18.HA has two functions. Firstly, it allows the recognition of target vertebrate cells, accomplished through the binding to these cells' sialic acid-containing receptors. Secondly, once bound it facilitates the entry of the viral genome into the target cells by causing the fusion of the host endosomal membrane with the viral membrane. The influenza virus Hemagglutinin (HA) protein is translated in cells as a single protein, HA, or hemagglutinin precursor protein. For viral activation, hemagglutinin precursor protein (HA) must be cleaved by a trypsin-like serine endoprotease at a specific site, normally coded for by a single basic amino acid (usually arginine) between the HA1 and HA2 domains of the protein. After cleavage, the two disulfide-bonded protein domains produce the mature form of the protein subunits as a prerequisite for the conformational change necessary for fusion and hence viral infectivity.
Storage Condition	Aliquot and store at -20℃ to -80℃. Avoid repeated freezing and thawing cycles.
Shipping Condition	In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. If supplied as liquid, the proteins will be shipped with dry ice.

FAQ

Q1-Spike ECD蛋白的主要功能是什么？

该蛋白包含OC43冠状病毒的受体结合域（RBD），识别宿主细胞表面的9-O-乙酰化唾液酸受体，介导病毒入侵呼吸道上皮细胞。
Q2-该蛋白能否用于广谱抗冠状病毒抗体研究？

是的，OC43与HKU1等β冠状病毒Spike蛋白具有部分保守表位，可用于筛选交叉中和抗体。
Q3-如何验证其天然三聚体结构？

通过凝胶过滤层析（SEC）和负染电镜观察，确认其以三聚体形式存在，模拟病毒表面构象。
Q4-His标签是否影响受体结合活性？

经表面等离子共振（SPR）检测，标签位于C端，不影响与宿主受体的亲和力（KD值验证）。
Q5-储存条件有何建议？

-80℃保存，避免反复冻融；溶解后4℃稳定3天，长期使用需分装冻存。

推荐产品

400-621-6806
614353794
周一至周五 9:00-18:00
0