HCoV-HKU1 (isolate N5) Spike Protein(ECD), His Tag_天津卡梅德科技发展有限公司

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HCoV-HKU1 (isolate N5) Spike Protein(ECD), His Tag

HCoV-HKU1 (isolate N5) Spike Protein(ECD), His Tag

产品编号

KMP4827

别名

人类冠状病毒刺突蛋白, HCoV-HKU1 Spike, S Protein, HCoV-HKU1 Spike Protein

规格

50ug
100ug
200ug

DATASHEET MSDS

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产品介绍

Alias	人类冠状病毒刺突蛋白, HCoV-HKU1 Spike, S Protein, HCoV-HKU1 Spike Protein
Catalog Number	KMP4827
Product Description	The HCoV-HKU1 (isolate N5) (HCoV-HKU1) Spike Protein(KMP4827) is produced in Insect Cells. A DNA sequence encoding the human coronavirus HKU1 (isolate N5) (HCoV-HKU1) spike protein (S1+S2 ECD) (Q0ZME7.1) (Met1-Pro1295) was expressed with a polyhistidine tag at the C-terminus. The purified protein was biotinylated in vitro.
Molecular Name	HCoV-HKU1 Spike
Species	HCoV-HKU1
Host	Insect Cells
Size	50ug, 100ug, 200ug
Purity	>95% as determined by SDS-PAGE
Purification	Affinity purification
Endotoxin	<1.0 EU/ug determined by the LAL method
Formulation	20mM Tris, 500mM NaCl, 10% Glycerol, pH7.5
Background	The influenza viral Hemagglutinin (HA) protein is a homotrimer with a receptor binding pocket on the globular head of each monomer.HA has at least 18 different antigens. These subtypes are named H1 through H18.HA has two functions. Firstly, it allows the recognition of target vertebrate cells, accomplished through the binding to these cells' sialic acid-containing receptors. Secondly, once bound it facilitates the entry of the viral genome into the target cells by causing the fusion of the host endosomal membrane with the viral membrane. The influenza virus Hemagglutinin (HA) protein is translated in cells as a single protein, HA, or hemagglutinin precursor protein. For viral activation, hemagglutinin precursor protein (HA) must be cleaved by a trypsin-like serine endoprotease at a specific site, normally coded for by a single basic amino acid (usually arginine) between the HA1 and HA2 domains of the protein. After cleavage, the two disulfide-bonded protein domains produce the mature form of the protein subunits as a prerequisite for the conformational change necessary for fusion and hence viral infectivity.
Storage Condition	Aliquot and store at -20℃ to -80℃. Avoid repeated freezing and thawing cycles.
Shipping Condition	In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. If supplied as liquid, the proteins will be shipped with dry ice.

FAQ

Q1-HKU1 Spike蛋白的临床意义是什么？

HKU1可引起儿童呼吸道感染，其Spike研究有助于理解季节性冠状病毒的传播机制。
Q2-该蛋白是否保留膜融合活性？

需与S2亚基共表达激活融合，单独ECD仅用于受体结合研究。
Q3-能否用于抗体中和实验？

是的，结合假病毒系统可评估抗体对病毒入侵的抑制效果。
Q4-表达系统是否影响蛋白折叠？

采用HEK293细胞表达，确保正确糖基化和构象。
Q5-如何避免蛋白降解？

添加1 mM EDTA和蛋白酶抑制剂，分装后-80℃保存。

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