HCoV-NL63 Spike Protein(ECD), His Tag_天津卡梅德科技发展有限公司

>>

>>

>>

>

HCoV-NL63 Spike Protein(ECD), His Tag

HCoV-NL63 Spike Protein(ECD), His Tag

产品编号

KMP4825

别名

人类冠状病毒刺突蛋白, HCoV-NL63 Spike, S Protein, HCoV-NL63 Spike Protein

规格

50ug
100ug
200ug

DATASHEET MSDS

立即询价加入询价列表大包装定制

产品介绍

Alias	人类冠状病毒刺突蛋白, HCoV-NL63 Spike, S Protein, HCoV-NL63 Spike Protein
Catalog Number	KMP4825
Product Description	The HCoV-NL63 (HCoV-NL63) Spike Protein(KMP4825) is produced in Insect Cells. A DNA sequence encoding the human coronavirus (HCoV-NL63) spike protein (S1+S2 ECD) (APF29071.1) (Met1-Pro1296) was expressed with a polyhistidine tag at the C-terminus. The purified protein was biotinylated in vitro.
Molecular Name	HCoV-NL63 Spike
Species	HCoV-NL63
Host	Insect Cells
Size	50ug, 100ug, 200ug
Purity	>95% as determined by SDS-PAGE
Purification	Affinity purification
Endotoxin	<1.0 EU/ug determined by the LAL method
Formulation	PBS, pH7.4
Background	The influenza viral Hemagglutinin (HA) protein is a homotrimer with a receptor binding pocket on the globular head of each monomer.HA has at least 18 different antigens. These subtypes are named H1 through H18.HA has two functions. Firstly, it allows the recognition of target vertebrate cells, accomplished through the binding to these cells' sialic acid-containing receptors. Secondly, once bound it facilitates the entry of the viral genome into the target cells by causing the fusion of the host endosomal membrane with the viral membrane. The influenza virus Hemagglutinin (HA) protein is translated in cells as a single protein, HA, or hemagglutinin precursor protein. For viral activation, hemagglutinin precursor protein (HA) must be cleaved by a trypsin-like serine endoprotease at a specific site, normally coded for by a single basic amino acid (usually arginine) between the HA1 and HA2 domains of the protein. After cleavage, the two disulfide-bonded protein domains produce the mature form of the protein subunits as a prerequisite for the conformational change necessary for fusion and hence viral infectivity.
Storage Condition	Aliquot and store at -20℃ to -80℃. Avoid repeated freezing and thawing cycles.
Shipping Condition	In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. If supplied as liquid, the proteins will be shipped with dry ice.

FAQ

Q1-Spike ECD的功能是什么？

ECD（胞外域）包含受体结合域（RBD），识别宿主ACE2受体，介导病毒入侵呼吸道上皮细胞。
Q2-NL63与其他冠状病毒的交叉免疫反应如何？

其Spike蛋白与SARS-CoV-2 RBD无显著交叉反应，但可研究广谱抗冠状病毒抗体。
Q3-His标签的纯化效率如何？

镍柱纯化后纯度≥90%，适用于ELISA包被或免疫原制备。
Q4-能否用于假病毒包装？

是的，与慢病毒载体结合可生成假病毒，用于中和抗体检测。
Q5-是否形成天然三聚体？

经凝胶过滤层析（SEC）验证，以三聚体形式存在，保留天然构象。

推荐产品

400-621-6806
614353794
周一至周五 9:00-18:00
0