HTNV (strain 84FLi) NP Protein, His Tag_天津卡梅德科技发展有限公司

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HTNV (strain 84FLi) NP Protein, His Tag

HTNV (strain 84FLi) NP Protein, His Tag

产品编号

KMP4823

别名

汉滩病毒核蛋白, HTNV NP, Nucleoprotein, HTNV NP Protein

规格

50ug
100ug
200ug

DATASHEET MSDS

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产品介绍

Alias	汉滩病毒核蛋白, HTNV NP, Nucleoprotein, HTNV NP Protein
Catalog Number	KMP4823
Product Description	The HTNV (strain 84FLi) NP Protein(KMP4823) is produced in Insect Cells. A DNA sequence encoding the hantaan virus (strain 84FLi) NP (AAK66740.1) (Met1-Leu429) was expressed with a polyhistidine tag at the C-terminus.
Molecular Name	HTNV NP
Species	HTNV
Host	Insect Cells
Size	50ug, 100ug, 200ug
Purity	>90% as determined by SDS-PAGE
Purification	Affinity purification
Endotoxin	<1.0 EU/ug determined by the LAL method
Formulation	20mM Tris, 10% Glycerol, 300mM NaCl, pH8.0
Background	The influenza viral Hemagglutinin (HA) protein is a homotrimer with a receptor binding pocket on the globular head of each monomer.HA has at least 18 different antigens. These subtypes are named H1 through H18.HA has two functions. Firstly, it allows the recognition of target vertebrate cells, accomplished through the binding to these cells' sialic acid-containing receptors. Secondly, once bound it facilitates the entry of the viral genome into the target cells by causing the fusion of the host endosomal membrane with the viral membrane. The influenza virus Hemagglutinin (HA) protein is translated in cells as a single protein, HA, or hemagglutinin precursor protein. For viral activation, hemagglutinin precursor protein (HA) must be cleaved by a trypsin-like serine endoprotease at a specific site, normally coded for by a single basic amino acid (usually arginine) between the HA1 and HA2 domains of the protein. After cleavage, the two disulfide-bonded protein domains produce the mature form of the protein subunits as a prerequisite for the conformational change necessary for fusion and hence viral infectivity.
Storage Condition	Aliquot and store at -20℃ to -80℃. Avoid repeated freezing and thawing cycles.
Shipping Condition	In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. If supplied as liquid, the proteins will be shipped with dry ice.

FAQ

Q1-NP蛋白在汉坦病毒中的功能是什么？

NP（核衣壳蛋白）包裹病毒RNA，参与基因组复制和免疫逃逸，是病毒颗粒组装的核心成分。
Q2-84FLi毒株的研究意义是什么？

该毒株分离自汉坦病毒出血热患者，研究其NP蛋白有助于开发血清学诊断试剂和疫苗。
Q3-His标签是否影响NP的抗原性？

经ELISA验证，His标签不影响NP与患者血清抗体的结合能力，适用于抗体检测。
Q4-该蛋白能否用于病毒载量检测？

是的，可通过qRT-PCR结合NP抗体检测临床样本中的病毒RNA-NP复合物。
Q5-储存条件如何？

-80℃保存，避免反复冻融；溶解后4℃稳定1周，长期使用建议分装冻存。

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