HIV (group N, strain 06CM-U14296) gp160 Protein, His Tag_天津卡梅德科技发展有限公司

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HIV (group N, strain 06CM-U14296) gp160 Protein, His Tag

HIV (group N, strain 06CM-U14296) gp160 Protein, His Tag

产品编号

KMP4809

别名

人类免疫缺陷病毒包膜蛋白gp160, HIV gp160, Envelope Protein gp160, HIV gp160 Protein

规格

50ug
100ug
200ug

DATASHEET MSDS

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产品介绍

Alias	人类免疫缺陷病毒包膜蛋白gp160, HIV gp160, Envelope Protein gp160, HIV gp160 Protein
Catalog Number	KMP4809
Product Description	The HIV (group N, strain 06CM-U14296) gp160 Protein(KMP4809) is produced in HEK293 Cells. A DNA sequence encoding the HIV-1 Envelope glycoprotein gp160 protein (Trp32-Arg504) was expressed with a polyhistidine tag at the C-terminus.
Molecular Name	HIV gp160
Species	HIV
Host	HEK293 Cells
Size	50ug, 100ug, 200ug
Purity	>95% as determined by SDS-PAGE
Purification	Affinity purification
Endotoxin	<1.0 EU/ug determined by the LAL method
Formulation	PBS, pH7.4
Background	The influenza viral Hemagglutinin (HA) protein is a homotrimer with a receptor binding pocket on the globular head of each monomer.HA has at least 18 different antigens. These subtypes are named H1 through H18.HA has two functions. Firstly, it allows the recognition of target vertebrate cells, accomplished through the binding to these cells' sialic acid-containing receptors. Secondly, once bound it facilitates the entry of the viral genome into the target cells by causing the fusion of the host endosomal membrane with the viral membrane. The influenza virus Hemagglutinin (HA) protein is translated in cells as a single protein, HA, or hemagglutinin precursor protein. For viral activation, hemagglutinin precursor protein (HA) must be cleaved by a trypsin-like serine endoprotease at a specific site, normally coded for by a single basic amino acid (usually arginine) between the HA1 and HA2 domains of the protein. After cleavage, the two disulfide-bonded protein domains produce the mature form of the protein subunits as a prerequisite for the conformational change necessary for fusion and hence viral infectivity.
Storage Condition	Aliquot and store at -20℃ to -80℃. Avoid repeated freezing and thawing cycles.
Shipping Condition	In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. If supplied as liquid, the proteins will be shipped with dry ice.

FAQ

Q1-gp160与gp120有何关系？

gp160是未切割的包膜前体蛋白，包含gp120和gp41，需宿主蛋白酶切割后才具有感染活性。
Q2-组N病毒的流行病学意义是什么？

组N是HIV-1的罕见分支，主要在中非地区发现，研究其gp160有助于追踪病毒起源与进化。
Q3-该蛋白能否用于假病毒包装？

是的，与HIV骨架质粒共转染可生成假病毒，用于中和实验或药物筛选（需生物安全审批）。
Q4-如何避免gp160的自发切割？

在哺乳动物细胞中表达时添加弗林蛋白酶抑制剂（如Dec-RVKR-CMK），维持前体形式。
Q5-是否提供糖基化分析数据？

可提供质谱鉴定的N-糖基化位点图谱（需额外订购）。

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