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HIV (group M, subtype B, isolate HXB2) Gag-Pol polyProtein, His Tag

HIV (group M, subtype B, isolate HXB2) Gag-Pol polyProtein, His Tag

产品编号

KMP4804

别名

人类免疫缺陷病毒Gag-Pol多聚蛋白, HIV Gag-Pol polyProtein, Gag-Pol poly, HIV Gag-Pol polyProtein Protein

规格
  • 50ug
  • 100ug
  • 200ug
产品介绍
Alias人类免疫缺陷病毒Gag-Pol多聚蛋白, HIV Gag-Pol polyProtein, Gag-Pol poly, HIV Gag-Pol polyProtein Protein
Catalog NumberKMP4804
Product DescriptionThe HIV (group M, subtype B (isolate HXB2) Gag-Pol polyProtein(KMP4804) is produced in E. coli. A DNA sequence encoding the p66 (Reverse Transcriptase + RNase H) of human immunodeficiency virus 1 gag-pol (NP_057849.4) (Pro588-Leu1147) was expressed with a polyhistidine tag at the N-terminus.
Molecular NameHIV Gag-Pol polyProtein
SpeciesHIV
HostE. coli
Size50ug, 100ug, 200ug
Purity>95% as determined by SDS-PAGE
PurificationAffinity purification
Endotoxin<1.0 EU/ug determined by the LAL method
FormulationPBS, pH7.4
BackgroundThe influenza viral Hemagglutinin (HA) protein is a homotrimer with a receptor binding pocket on the globular head of each monomer.HA has at least 18 different antigens. These subtypes are named H1 through H18.HA has two functions. Firstly, it allows the recognition of target vertebrate cells, accomplished through the binding to these cells' sialic acid-containing receptors. Secondly, once bound it facilitates the entry of the viral genome into the target cells by causing the fusion of the host endosomal membrane with the viral membrane. The influenza virus Hemagglutinin (HA) protein is translated in cells as a single protein, HA, or hemagglutinin precursor protein. For viral activation, hemagglutinin precursor protein (HA) must be cleaved by a trypsin-like serine endoprotease at a specific site, normally coded for by a single basic amino acid (usually arginine) between the HA1 and HA2 domains of the protein. After cleavage, the two disulfide-bonded protein domains produce the mature form of the protein subunits as a prerequisite for the conformational change necessary for fusion and hence viral infectivity.
Storage ConditionAliquot and store at -20℃ to -80℃. Avoid repeated freezing and thawing cycles.
Shipping ConditionIn general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. If supplied as liquid, the proteins will be shipped with dry ice.

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